Study on Tissue Culture of Sweet Broad Pea

Hui Fang Chi

doi:10.4028/www.scientific.net/AMM.644-650.5407

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 644-650Study on Tissue Culture of Sweet Broad Pea

Study on Tissue Culture of Sweet Broad Pea

Abstract:

s. The cotyledons, Internodes, leaves and stems of sweet broad pea were studied on tissue culture. Research results show that: The ability of different explants for callus formation and adventitious bud differentiation in different culture medium is different. The callus formation rate and sprouting rate of Internodes is significantly higher than other explants, which is a ideal material for tissue culture. The callus formation rate of Internodes was 100% in MS +BA1.0 mg/L+NAA 1.0 mg/L and MS+ 2, 4-D 0.5 mg/L; The bud differentiation is best at the medium of MS+ 6-BA 2 mg/L, which reached 86.7%; the rooting rate was 83.3% at the medium of MS+ NAA 3mg/L. The study provides a experimental basis for further study on the plant regeneration in the sweet broad pea.

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Periodical:

Applied Mechanics and Materials (Volumes 644-650)

Pages:

5407-5410

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.644-650.5407

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Online since:

September 2014

Authors:

Hui Fang Chi*

Keywords:

Explants, Plant Regeneration, Sweet Broad Pea, Tissue Culture

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References

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