Effects of Temperature on Mixed-Culture Fermentative Hydrogen Production from Corn Stover Hydrolyzate

Abstract:

Four commonly-used temperatures (37°C, 30°C, 55°C, and 70°C) were examined on mixed-culture H₂-producing behavior with corn stover hydrolyzate as the substrate. Experimental results indicated that the optimum biogas (1505.7 mL/L) and H₂ (717.2 mL/L) production was achieved at 55°C. For soluble metabolites, the highest acetate/butyrate concentrations and the lowest ethanol production were also found at 55°C. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was employed to analyze corresponding microbial community structure. At 55°C, most bacteria were identified as Clostridium spp., which might be responsible for higher H₂ yield in this case. Totally, fermentation temperation of 55°C was probably preferable for H₂ production from corn stover hydrolyzate.

• Periodical: Applied Mechanics and Materials (Volumes 672-674)
• Pages: 159-163
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.672-674.159
• Online since: October 2014
• Authors: Kun Zhang*, Nan Qi Ren
• Keywords: Biohydrogen, Corn Stover Hydrolyzate, Microbial Community, Temperature

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