The Influence of Ecological Remediation Process on Bacterial Community Composition in Beishi River Sediment Determined by PCR-DGGE Analysis

Ji Hua Wang; Jian Fei Guan; Xue Chen Yang; Miao Wang; Shan Shan Zhang; Wan Tong Gu

doi:10.4028/www.scientific.net/AMM.675-677.102

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 675-677The Influence of Ecological Remediation Process on...

The Influence of Ecological Remediation Process on Bacterial Community Composition in Beishi River Sediment Determined by PCR-DGGE Analysis

Abstract:

Pollution of urban river is a major risk to human health and water quality throughout the world. The purpose of this study was to determine the influence of ecological remediation process on sediment bacterial community structure. Three sediment samples in urban river located in Changzhou city, the bacterial community structure was studied in the process of ecological remediation period using denaturing gradient gel electrophoresis (DGGE). The results of the microbial community analysis were related to water environmental parameters through the analysis to investigate the relationship between potential impact of water quality and microbial community structure. As the ecological remediated river, T, DO and COD were the important environmental variables influencing the sediment microbial community composition. Microbial community changes determined by DGGE patterns showed that microbial community structures in the same period did not change further, while those in different period changed continuously. In July 2010, Pseudomonas sp, Escherichia sp, Exiguobacterium sp and Bacillus sp as each point jointly owned. In October 2010, Acinetobacter sp, Clostridiales, Dechloromonas sp and uncultured bacterium were belonged to these three sample sites.