Cloning of MeGolS5 Promoter from Cassava (Manihot esculenta Crantz) and Expression Analysis in Abiotic Stress of MeGolS5

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The MeGolS5 promoter fragment (1492 bp) was amplified from the genomic DNA of Manihot esculenta Crantz by inverse polymerase chain reaction. Promoter sequence analysis by PLACE and PlantCARE showed that the cloned fragment contained several putative cis-elements, such as abscisic acid response element (ABRE), heat shock elements (HSE), as well as TATA-Box and CAAT-Box. The expression prfile of MeGolS5 shows that the gene is induced by several abiotic stresses, such as salt, drought, H2O2 and ABA.

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126-132

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December 2014

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© 2015 Trans Tech Publications Ltd. All Rights Reserved

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