Identification and Photodegradation of Red Pigment from Serratia marcesce zl3

Lin Zhou; Shuang Zhu; Xin Huai Dong

doi:10.4028/www.scientific.net/AMM.737.349

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 737Identification and Photodegradation of Red Pigment...

Identification and Photodegradation of Red Pigment from Serratia marcesce zl3

Abstract:

Present study was aimed to identify and isolate the red pigment from Serratia marcesce zl3, and to investigate the factors affecting on the light stability of the purified red pigment. Silica gel and Sephadex LH-20 column chromatography were used for purification, identification was done by TLC, UV, FTIR and HPLC-MS techniques. The effect of pH and light intensity on the light stability of the purified red pigment prodigiosin was analyzed by TLC, UV and HPLC. The stability of identified prodigiosin pigment at pH 1, 3, 5 was superior to that at pH 7, 10, 14 according to the determination of OD₅₃₅. Significant level of degradation of the prodigiosin was observed for 24 h, using 10 klx continuous white light. The effect of pH to the stability of prodidgiosin was partly reversible, while the degradation of prodigiosin under 4, 10, 20 klx white light was irreversible.

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Applied Mechanics and Materials (Volume 737)

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349-352

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.737.349

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Online since:

March 2015

Authors:

Lin Zhou*, Shuang Zhu, Xin Huai Dong

Keywords:

Identification, Photodegradation, Prodigiosin, Purification

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