Study on Process of Xylanase by Fed-Batch Fermentation

Abstract:

Xylanase has good application prospect in the paper and pulp industry, feed industry, health care and bioenergy industry,etc.The xylanase is one of the key enzyme in the utilization of biomass. Fermentation kinetics equation was constructed based on Logistic and Luedeking-Piret equation. The results showed that the dissolved oxygen was related to the stirring speed, raising to 45% naturally after the log growth phase (about 30h) on the base of the optimum medium process of xylanase production by Trichoderma reesei. Fed-batch fermentation strategy was designed based on fermentation kinetics equations. The xylanase activity was further enhanced to 2406.175U/mL, enzyme activity per unit protein mass was 6573U/mg.

• Periodical: Applied Mechanics and Materials (Volume 737)
• Pages: 381-387
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.737.381
• Online since: March 2015
• Authors: Mei Zhen Gong, Rui Huang, Jin Ling Guo, Hua Shun Yu, Juan Yao, Da Chun Gong*
• Keywords: Fed-Batch Fermentation, Process, Xylanase

References

[1] F. Gwen, L . Katerina: App. Envir. Microbio. Vol. 75 (2009), p.454.

[2] C. Sophie, M. Sophie, S. Jean : Am. J. Eno. Vitic, Vol. 56 (2005), p.238.

[3] H. Xiong, N. Weymarn, M. Leisola: Process Biochemistry, Vol. 39 (2004), p.731.

[4] A. Oliveira, A. Porto: Bioresource Technology, Vol. 97 (2006), p.862.

[5] D.A. Bocchini, O. M. Oliveira: Process Biochemistry, Vol. 40(2005), p.3653.

[6] H. WAN, i W. JIA , H. CAI: Science and Technology ofFood Industry, Vol. 29(2009), p.108.

[7] L. MAO , Q. YONG , C. YAO S. YU: Modern Chemical Industry, Vol. 25(2005): p.151.

[8] H. Ming, D. Song,Y. Liu,G. Nie: South west China Journal of Agricultural Science, Vol. 22(2009), p.1023.

[9] Q. YONG, M. CHEN, Y. JING: Chemistry and Industry of ForestProducts , Vol. 16(2013), p.6.

[10] Z. LIU , Y., CHEN, W. QI: Chemical Engineering, Vol. 36(2008), p.66.