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Building Up of Surface Plasmon Resonance Instrument Using Spreeta 2000 and Arduino Mega 2560
Abstract:
A system to monitor the signal produced by surface plasmon resonance (SPR) has been designed and tested. The built-in sensor consists of a polarized light source having a wave length 840 nm and a built in 128 photodiodes constructed around the maximum SPR angle to detect the reflection light from the gold surface. The signal produced from the diodes is then amplified and digitized. We use 5173 Hz clock to sample the signal generated by the diode array pixel during the reflection process producing SPR signal around the maximum angle. We program Arduino Mega 2560 to process the signals and transfer it using Lab View to present the data versus sampling time. Doing this, we can record the SPR signal versus time, known as the SPR dynamic signal. To test the performance of the system and to know the properties of the SPR signal, we test the system using ethyl alcohol with concentration of 10% (v/v) up to 90% (v/v). The result shows that the system can be used to monitor the dynamics of SPR signal during the reaction process and it indicates the possibility of using the instrument to detect various kinds of SPR signal related to the chemical and or biological process in the samples.
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25-28
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July 2015
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© 2015 Trans Tech Publications Ltd. All Rights Reserved
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