A Novel Electrochemical Immunosensor Based on DNA/Fe3O4 (Core) /ZrO2（Shell）one Dimension Magnetic Probes for Carcino-Embryonic Antigen
A novel and sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) system of Hydroquinones (HQ)- peroxide hydantoin (CP) -horseradish-peroxidase (HRP) by using magnetic DNA linked core-shell Fe3O4@ZrO2（ZMPs）nanoparticles as labels and signal amplification was established. In the presence of carcinoembryonic antigen (CEA) analyte, the sandwich-type immunocomplex could be formed between HRP labeled CEA second antibody（CEA Ab2） modified DNA-ZMPs probes (DNA / (ZMPs-HRP-CEA Ab2) n) and nano-gold CEA modified glassy carbon electrode (GCE | CEA Ab1) as an immune electrode.The concentration of CEA was determined based on the current signal, which was generated in the reaction between HQ and CP catalyzed by HRP on the sandwich-type immunocomplex. ZMPs nanoparticles as label material can not only perform the rapid separation and purification of signal antibody on magnetic field, but also immensely enhance the labeled capacity of HRP-anti-CEA which can amplify the catalytic current signal. Furthermore, the electrode modification process was not required. The method provided a linear response range between 0.01 and 200 ng·mL-1 with a detection limit of 4 pg·mL-1. Moreover, the proposed electrochemical ELISA method exhibited good precision, high sensitivity and acceptable reproducibility, and could be further developed for clinical detections of CEA and other biomarkers.
Linli Xu, Wenya Tian and Elwin Mao
F. T. Hu et al., "A Novel Electrochemical Immunosensor Based on DNA/Fe3O4 (Core) /ZrO2（Shell）one Dimension Magnetic Probes for Carcino-Embryonic Antigen", Applied Mechanics and Materials, Vols. 80-81, pp. 200-206, 2011