A Novel Electrochemical Immunosensor Based on DNA/Fe₃O₄ (Core) /ZrO₂（Shell）one Dimension Magnetic Probes for Carcino-Embryonic Antigen

Abstract:

A novel and sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) system of Hydroquinones (HQ)- peroxide hydantoin (CP) -horseradish-peroxidase (HRP) by using magnetic DNA linked core-shell Fe₃O₄@ZrO₂（ZMPs）nanoparticles as labels and signal amplification was established. In the presence of carcinoembryonic antigen (CEA) analyte, the sandwich-type immunocomplex could be formed between HRP labeled CEA second antibody（CEA Ab2） modified DNA-ZMPs probes (DNA / (ZMPs-HRP-CEA Ab2) n) and nano-gold CEA modified glassy carbon electrode (GCE | CEA Ab1) as an immune electrode.The concentration of CEA was determined based on the current signal, which was generated in the reaction between HQ and CP catalyzed by HRP on the sandwich-type immunocomplex. ZMPs nanoparticles as label material can not only perform the rapid separation and purification of signal antibody on magnetic field, but also immensely enhance the labeled capacity of HRP-anti-CEA which can amplify the catalytic current signal. Furthermore, the electrode modification process was not required. The method provided a linear response range between 0.01 and 200 ng·mL^-1 with a detection limit of 4 pg·mL^-1_. Moreover, the proposed electrochemical ELISA method exhibited good precision, high sensitivity and acceptable reproducibility, and could be further developed for clinical detections of CEA and other biomarkers.

• Periodical: Applied Mechanics and Materials (Volumes 80-81)
• Pages: 200-206
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.80-81.200
• Online since: July 2011
• Authors: Fu Tao Hu, Yuan Zao Wu, Yu Ting Cao, Ning Gan
• Keywords: Carcinoembryonic Antigen, DNA, Fe₃O₄/ZrO₂, One Dimension Magnetic Nanoprobe, Sandwich Electrochemical Immunosensor

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