Performance of some New Bacterial Isolates on Biodegradation of Libyan Light Crude Oil Using Agro-Industrial Wastes as Co-Substrates

Abstract:

Some unfortunate accidents of large amount of oil hydrocarbons have been reported in Mediterranean Sea which has caused severe environmental damage to the area. To safeguard the Libyan coastline and marine ecologysince thousands of human activity such as fishing largely depends on the healthycoastal environment.Twenty crude oil-degrading bacterial isolates were obtained from oil-contaminated sites at Al Hariga Oil Terminal and Nafoora Oilfield. Based on a high growth rate in crude oil and hydrocarbon degradation efficiency, two isolates were selected from the twenty isolates for further analysis. The nucleotide sequence of 16S rRNA gene showed that these isolates are likely Pseudomonas aeruginosa andKocuriapastrius. One of the isolates is a potential Gram-negative Pseudomonas bacterium based on petroleum hydrocarbon degradation efficiency and potent emulsifying activity; thus, this isolate is identified as P. aeruginosa NAF1; the other isolate K. pastrius SAR3 is identified as a Gram-positive bacterium. Corn steep liquor (CSL) and solid waste date (SWD) were used for an in situ molasses technique to enhance bacterial growth and biodegradation efficiency. P. aeruginosa NAF1 exhibited 70% and 76% crude oil degradation in 0.2% (w/v) CSL and SWD in 28 d, respectively. Likewise, K. pastrius SAR3 yielded 68% and 70% crude oil degradation in 0.2% (w/v) CSL and SWD in 28 d, respectively.

• Periodical: Applied Mechanics and Materials (Volume 802)
• Pages: 496-500
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.802.496
• Online since: October 2015
• Authors: Abdullah M. El Mahdi*, Hamidi Abdul Aziz, Salem S. Abu Amr, Nour Sh. El-Gendy, Hussein Nassar
• Keywords: 16S rDNA, Biodegradation, Crude Oil, Identification, Isolation

References

[1] M. Soltani, Distribution lipidique et voiesmétaboliques chez quatrebactéries Gram-négatives hydrocarbonoclastes. Variation en fonction de la source de carbone. PhD diss., Chimie Paris Tech, (2004).

[2] S.Y.T. Adeline, H.C.T. Carol and C.S. Aw, Hydrocarbon-degradation by isolate Pseudomonas lundensis UTARFPE2. Malaysian J. Microbiol. 5(2) (2009) 104-108.

DOI: 10.21161/mjm.15609

[3] M. Vidali, Bioremediation An overview, Pure Appl. Chem., 73 (2001) 1163–1172.

[4] S. Harayama, Y. Kasai and A. Hara, Microbial communities in oil-contaminated seawater, Curr. Opin. Biotechnol., 15 (2004) 205-214.

DOI: 10.1016/j.copbio.2004.04.002

[5] C.S. Piddington, B.R. Kovacevich and J. Rambosek, Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulfurization operon of Rhodococcus sp. strain GTS8. Appl. Environ. Microbiol., 61(2) (1995) 468‏-475.

DOI: 10.1128/aem.61.2.468-475.1995

[6] H.J. Benson, Microbiological applications, 6th ed., Wm. C. Brown Publishers‏, (1994) p.447.

[7] D.R. Boone, Castenholz, G. M. Garrity, D. J. Brenner, N. R. Krieg and J. T. Staley, (Eds. ). Bergey's R. W. Manual® of Systematic Bacteriology, Springer Science & Business Media, 2 (2005).

[8] R. S. Dhanve, D. C. Kalyani, S. S. Phugare and J. P. Jadhav, Coordinate action of exiguo bacterial oxido reductive enzymes in biodegradation of reactive yellow 84A dye, Biodegradation, 20(2) (2009) 245-255.

DOI: 10.1007/s10532-008-9217-z

[9] R. Thavasi, S. JayalakshmiI and M. Banat, Effect of biosurfactant and fertilizer on biodegradation of crude oil by marine isolates of Bacillus megaterium, Corynebacterium kutscheri and Pseudomonas aeruginosa. Bioresour. Technol., 102 (2011).

DOI: 10.1016/j.biortech.2010.08.099

[10] Y. Zhang, R. M. Miller, Effect of a Pseudomonas rhamnolipid (biosurfactant) on cell hydrophobicity and biodegradation of octadecane. Appl. Environ. Microbiol., 60 (1994) 2101 - 2106.

DOI: 10.1128/aem.60.6.2101-2106.1994

[11] L. Ruberto, S. Vázquez and M.W. Cormack, Effectiveness of the natural bacterial flora. Biostimulation and bioaugmentation on the bioremediation of a hydrocarbon contaminated antartic soil, Int. Biodeter. Biodegr., 52 (2003) 115-125.

DOI: 10.1016/s0964-8305(03)00048-9

[12] S. Sharma, H. Pathak. Pseudomonas in biodegradation., Int. J. Pure Appl. Biosci, 2 (2014) 213-222.

[13] W. Ismail, N. A. Alhamad, W. S. El-Sayed, A. M. El Nayal, Y. Chiang and R. Y. Hamzah, Bacterial Degradation of the Saturate Fraction of Arabian LightCrude oil: Biosurfactant Production and the Effect of ZnO Nanoparticles, J. Pet. Environ. Biotechnol., 4 (2013).

DOI: 10.4172/2157-7463.1000163