Optimization of HCO₃^- Production Reflect to CaСo₃ Precipitation for Self-Healing by Bacillus sphaericus

Abstract:

Bacteria are able to perform metabolic activities which promote the precipitation of calcium carbonate in the form of calcite. Bacillus Sphaericus was used in this study, which is an ureolytic bacteria that can precipitate calcium carbonate in its environment by the decomposition of urea into ammonium and carbonate. The bacterial degradation of urea basically increases the pH and promotes the microbial deposition of carbonate as calcium carbonate. In this research, the capability of bacteria to influence the formation of HCO₃^- by the production of urease enzyme was investigated. Results of growth rate and characteristics of bacteria showed that 20g/L of urea concentration was able to provide a good environment for bacteria with sufficient amount of nutrient to survive. The formation of HCO₃^- was parallel with NH₃ production where the formation of HCO₃^- increased slowly as the ammonia production decreased. Urea degradation with suitable concentration of urea by 20g/L may form high HCO₃^- compared to 25g/L urea concentration. The results from the experimental works indicated that the optimal urea concentration was 20g/L.

• Periodical: Applied Mechanics and Materials (Volume 802)
• Pages: 549-554
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.802.549
• Online since: October 2015
• Authors: Norfaniza Mokhtar, Zhameir Shafiq Mohd Ilias, Husnul Azan Tajarudin*, Megat Azmi Megat Johari
• Keywords: Bacillus sphaericus, HCO₃^-, Microbial Mineral Precipitation, Urease Activity

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