Antioxidant Activities of Transgenic Flower Over-Expressing FLS and TT8 Involving in Flavonoid Biosynthesis

Abstract:

Transgenic differently over-expressed cDNA involving in flavonoid biosynthesis (FLAVONOL SYNTHASE: FLS and TRANSPARENT TESTA 8: TT8) and wild type plants were grown in the culturing room radiated with fluorescent light. Flowers of each transgenic and WT were collected and extracted with acidic methanol. The flower extracts were analyzed for the relative content of each flavonoid subgroup by spectrophotometry. The result showed that the both transgenic extracts contained flavanone and flavone higher than WT extract did. The FLS extract contained the highest amount of flavonol content (1.4 times) following with the TT8 extracts (1.25 times) comparing to WT extract. The TT8 extract has anthocyanin content approximately 3 times, while the FLS extract has only 0.5 times of WT. The extract was used to evaluate the antioxidant capacity by ABTS and DPPH methods. The result showed that, both transgenic extracts have a better antioxidant capacity than WT extract. Both transgenic extracts have similar results of EC50 performed by DPPH method, however the TT8 extracts showed the highest antioxidant capacity under ABTS experimental condition. Therefore, types and concentration of flavonoids in the extract could directly affect the antioxidant capacity.

• Periodical: Applied Mechanics and Materials (Volume 879)
• Pages: 78-82
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.879.78
• Online since: March 2018
• Authors: Darin Dangrit, Kanokporn Sompornpailin*
• Keywords: ABTS, Antioxidants, Biomaterials, DPPH, Flavonoids, Flavonols

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