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Protein Biomarkers from Physiological Disorder Syndrome in Leave, Peel and Seed of Longan on Fruit Growth Using One-Dimension Electrophoresis Technique Followed by Protein Identification with LC-MS/MS

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Abstract:

Proteomic investigation of leaves, peels and seeds on longan (Dimocarpus longan Lour. cv. Daw) at Maejo University’s farm in Chiang Mai province, Thailand. There were comparisons between normal and physiological disorder syndromes in longan on fruit growth (5, 10, 15, 20, 25 and 30 weeks fruition, respectively) by one dimensional electrophoresis (1-D gel) technique at a concentration of 12.5% gel in reducing condition with Coomassie brilliant blue R-250 staining coupling on liquid chromatography tandem mass spectrometry (LC-MS/MS). The research showed that vegetative storage protein (25.2 KDa), a storage protein in plants, was expressed in leaves, peels and seeds on before and after physiological disorder syndrome occurring. A protein involved on photosynthesis, the ribulose-1,5-bisphosphate carboxylase/oxygenase (48.3 KDa), was represented only on leaves at 30 weeks after fruition but not in peels and seeds of longan. Two proteins, the BnaC01g20210 (35.8 KDa) and predicted protein (40.11 KDa), which could not be identified the type and function of the metabolism, were decreased in diseased longan. These proteins may be important protein in part of the recovery process of abnormal longan. Therefore, advanced technique will be used for further proteomic studies.

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Periodical:

Applied Mechanics and Materials (Volume 886)

Pages:

14-20

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.886.14

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Online since:

January 2019

Authors:

Ekawit Threenet*, Achara Kleawkla, Yossaporn Kaewkalong, Winai Wiriyaalongkorn, Adisak Joomwong, Theerawee Kleawkla, Thritsanat Kleawkla

Keywords:

Electrophoresis, Leaves, Longan, PEELS, Physiological Disorder Syndromes, Seeds

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© 2019 Trans Tech Publications Ltd. All Rights Reserved

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