Amperometric Biosensors Using Different Alcohol Oxidases

Abstract:

Amperometric biosensors were fabricated by immobilizing alcohol oxidases (AOX) from two different sources onto glutaraldehyde (GA)-activated supports. Alcohol oxidases from Hansenula sp. and from Pichia pastoris were employed for immobilization. The biosensor with AOX from Hansenula sp. showed a linear response to ethanol in the concentration range of 0.1-0.6 mM with a sensitivity of 88.534 µA mM^-1 cm^-2 and a detection limit of 0.1 mM (S/N=3). In comparison, the biosensor with AOX from P. pastoris showed a linear response from 0.1-0.5 mM ethanol with a sensitivity of 76.886 µA mM^-1 cm^-2 and a detection limit of 0.1 mM. The study of stability of biosensors revealed that after 90 measurements, the biosensor with AOX from Hansenula sp. retained 97% of its original current response whereas the current response of the biosensor with AOX from P. pastoris decreased to 81% of its initial value. The biosensor with AOX from Hansenula sp. demonstrated slightly higher sensitivity and stability than the biosensor with AOX from P. pastoris.

• Periodical: Applied Mechanics and Materials (Volume 891)
• Pages: 90-95
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.891.90
• Online since: May 2019
• Authors: Manatsapon Tipmanee, Saipin Thanachasai*
• Keywords: Alcohol Oxidase, Amperometric Biosensor, Ethanol

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