In order to assess the service states of the permanent prestress of transverse prestressing bundle for the Xiangjiang Yinpenling Bridge (XYB), the permanent prestress values are measured. Appearance census of the diseases of transverse prestressing bundles for the cable-stayed part, the east and west incremental launching girder parts of the XYB are carried out. Three transverse prestressing bundles of the most serious corrosion are investigated by windowing damage inspection. The measured results and the analysis of the permanent prestress for the XYB show that the average permanent prestress of the three transverse prestressing bundles is about 300MPa to 550MPa, which do not meet the requirements of the design tension stress. It suggests that the appropriate treatment measures should be taken.