The main form of interfacial bond stress between prestressed CFRP sheets and the beams of steel reinforced concrete(SRC) are interfacial shear and peel-off stress. The effective transfer of adhesive stress is the fundamental to ensure the structure bearing capacity improved after strengthened. According to the principle of minimum potential energy and combining with the mechanical behavior of prestressed CFRP sheets and the beams of SRC, the calculated formulas of interfacial shear and peel-off stress are deduced under symmetry concentrated loads. The influences of initial strain and the layer of CFRP sheets are discussed. The calculation results show that the effective availability of CFRP sheets is increased with the initial strain increasing under the load. And the interfacial shear and peel-off stress of beam-end are increased simultaneously with the initial strain and the layers of CFRP sheets increasing, which lead to the ends of beams occurred peel-off failure easily.