This research applies Negative Binomial regression models to investigate safety effects of ramp spacing. Data for model estimation was collected in 112 freeway segments where each entrance ramp is followed by an exit ramp. Three years (2005-2007) of freeway crash data were examined by the NB model in this study. The modeling results suggest that the frequencies of total crashes, fatal-plus-injury crashes, single-vehicle crashes and multiple-vehicle crashes increase as ramp spacing decreases, and the frequencies of total crashes and multiple-vehicle crashes increase at significant rates. The modeling result has been geared into the development of accident modification factors (AMFs) for ramp spacing that can be used safety prediction of freeways.