Effects of Elevated Carbon Dioxide on Soil Bacterial Community Structure

Abstract:

The soil bacteria diversity in different concentrations of CO₂ was investigated in the simulation test area, by using PCR (Polymerase Chain Reaction) -DGGE (Denaturing Gradient Gel Electrophoresis) and 16Sr-DNA library technology. The results showed that the bacteria diversity coefficient (P) got down to 0.8710, 0.8710 and 0.7742 from 0.9032 (from control area) when the concentration of CO₂ in soil reached 20000ppm, 40000ppm, 60000ppm respectively. With the increasing concentration of CO₂ in soil, the abundance of the original low-density bacteria such as Asticcacaulis excentricus, etc. increased, while the abundance of unclassified_Rhizobiales reduced significantly. Dominant bacteria such as Brucella melitensis etc. had the higher homology. It can be revealed that high concentration of CO₂ had a significant impact on the soil bacteria community diversity, while a weak influence on main bacterial species. Azotobacter was sensitive to the increasing of the CO₂ concentration. Great reducing of their abundance had an adverse effect on nitrogen-fixing capability of soil.

• Periodical: Advanced Materials Research (Volumes 1010-1012)
• Pages: 422-428
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1010-1012.422
• Online since: August 2014
• Authors: Chun Rong Li, Wen Ke Wang, Hong Zhang Deng, Xiao Hong Zhao, Feng Han, Rong Wang
• Keywords: Bacterial Community Structure, Carbon Dioxide, PCR-DGGE, Soil

References

[1] Houghton J T, Meirafilho L G, Callander B A, et al. 1996. Climate Change 1995, The Science of Climate Change. Cambridge University Press, 572.

[2] Hu J L, Lin X G, Chu H Y, Yi R, Zhang H Y, Yuan X X, Zhu J G, 2006. A review on soil microbial responses to elevated atmospheric CO2 concentration. Chinese Journal of Soil Science, 37 (3) : 601-605.

[3] Xu Z G, Chen D Z, Zeng R S, 2007. Geological storage of CO2 and commercial utilization. Advances in Earth Sciences, 22 (7) : 698-707.

[4] Xu G Q, Li Y, Shi Y, Huang G H, 2002. Effect of free-air CO2 enrichment on soil microbe in paddy field. Chinese Journal of Applied Ecology, 13 (10): 1358-1359.

[5] Bachu S, Gunter W D, Perkings E H, 1994. Aquifer disposal of CO2 hydrodynamic and mineral trapping. Energy Conversion and Management, 35 (4) : 269-279.

DOI: 10.1016/0196-8904(94)90060-4

[6] Minh H D, Rodica L, 2011. Actuarial risk assessment of expected fatalities attributable to carbon capture and storage in 2050. International Journal of Greenhouse Gas Control, 5, 1346-1358.

DOI: 10.1016/j.ijggc.2011.07.004

[7] White C, 2005. Sequestration of carbon dioxide in coal with enhanced coal bed methane recovery-a review. Energy Fuels, 19 (3) : 659-724.

[8] Hitchon B, Gunter W D, Gentzis T, Bailey R T, 1999. Sedimentary basins and greenhouse: a serendipitous association. Energy Conversion and Management, 40 : 825-843.

DOI: 10.1016/s0196-8904(98)00146-0

[9] Li X C, Liu Y F, Bai B, Fang Z M, 2006. Ranking and screening of CO2 saline aquifer storage zones in China. Journal of Rock Mechanics and Engineering, 25 (5) : 963-968.

[10] Zhang H T, Wen D G, Li Y L, Zhang J Q, LU J C, 2005. Conditions for CO2 geological sequestration in China and some suggestions. Geological Bulletin of China, 24 (12) : 1107-1110.

[11] Michael K, Golab A, Shulakova V Ennis-king J, Allinson G, Sharma S, Aiken T, 2010. Geological storage of CO2 in saline aquifers: A review of the experience from existing storge operations. International Journal of Greenhouse Gas Control, 4 : 659-667.

DOI: 10.1016/j.ijggc.2009.12.011

[12] Sun D W, 2011. For economic sustainable development-CO2 Storage 1000 meters underground by Shenhua Group Corporation Limited. China Petroleum and Chemical Industry, 08 : 11-15.

[13] Lee A M, 2003. The hutchinson gas explosions: unravelling a geologic mystery, kansas bar association [C]. 26th Annual KBA/KIOGA Oil and Gas Law Conference , 3-29.

[14] M. Cruz Payán, Bram Verbinnen, Berta Galan, Alberto Coz, Carlo Vandecasteele, Javier R, Viguri, 2012. Potential influence of CO2 release from a carbon capture storage site on release of trace metals from marine sediment. Environmental Pollution, 162, 29-39.

DOI: 10.1016/j.envpol.2011.10.015

[15] Bhawna Singh, Anders H, Strømman, Edgar G, 2012. Environmental damage assessment of carbon capture and storage. Journal of Industrial Ecology, 16 (3) : 407-419.

DOI: 10.1111/j.1530-9290.2012.00461.x

[16] Jill Wildaya, Mike Wardmana, Michael Johnsona, Mike Hainesb, 2011. Hazards from carbon dioxide capture, transport and storage. Process Safety and Environmental Protection, 89 : 482-491.

DOI: 10.1016/j.psep.2011.09.002

[17] Manal Al-Traboulsi, Sofie Sjogersten, Jeremy Colls , Michael Steven, Jim Craigon, Colin Black, 2012, Potential impact of CO2 leakage from carbon capture and storage (CCS) systems on growth and yield in spring field bean. Environmental and Experimental Botany, 80 : 43-53.

DOI: 10.1016/j.envexpbot.2012.02.007

[18] Xu Z G, Chen D Z, Zeng R S, 2008. The leakage risk assessment and remediation options of CO2 geological storage. Geological Review, 54 (3) : 373-386.

[19] Mou J, Sun B S, Chen Y, 2010. Microbial community structure in a membrane bioreactor determined using PCR-DGGE. Acta Scientiae Circumstantiae, 30 (4) : 729-734.

[20] Luo J F, Tian G L, Lin W T, 2013. Enrichment, isolation and identification of sulfur-oxidizing bacteria from sulfide removing bioreactor. Journal of Environmrntal Sciences, 25 (7) : 1397-1399.

DOI: 10.1016/s1001-0742(12)60179-x

[21] Sambrook J, Fritsch E F , Maniatis T , et al. Molecular Cloning: A laboratory Manual [M]. (2nd ed). Cold Spring Harbor Laboratory Press, (1989).

DOI: 10.1016/0167-7799(91)90068-s

[22] Zhang H W, Zhang Q R, Zhou Q X, et al. 2003. Introduction and progress of molecular microbial ecology. Journal of Applied Ecology, 12 (2) : 286-292.

[23] Thompson J D, Higgins D G, Gibson T J, Clustal W, 1994. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res, 22 (22) : 4673-4680.

DOI: 10.1093/nar/22.22.4673

[24] Jia X, Han S J, Zhao Y H, Zhou Y M, 2006. Response of bacterial community structure in Pinus Koraiensis seedlings rhizosphere and bulk soil to elevated CO2. Acta Scientiae Circumstantiae, 26 (11) : 1833-1837.

[25] Hodge A, Paterson E, Grayston S, et al. 1998. Characterisation and microbial utilization of exudates material from the rhizosphere of Lolium perenne grown under CO2 enrichment. Soil Biology& Biochemistry, 30 : 1033-1043.

DOI: 10.1016/s0038-0717(97)00269-1

[26] Delong E F, Wickham G S, Pace N R, 1989. Phylogenetic stain s ribosomal RNA-based probes for the identification of single cells. Science, 243 : 1360-1363.

DOI: 10.1126/science.2466341