Applied Technology in Using HPLC for Determining Aflatoxin Contents in Peanuts

Yong Gang Shi; Wei Zhong; Hao Wen; Ju Ma; Ping Sun

doi:10.4028/www.scientific.net/AMR.1022.76

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1022Applied Technology in Using HPLC for Determining...

Applied Technology in Using HPLC for Determining Aflatoxin Contents in Peanuts

Abstract:

Aflatoxins in grinded peanuts were extracted with 70% methanol and purified with immuno affinity column. In order to enhance the test sensitivity, the Aflatoxin was derived by trifluoroacetic acid. the derived Aflatoxin B1, B2, G1 and G2 were analyzed by HPLC with fluorescence detector. The determination could be completed in 14 minutes. The detect limits of Aflatoxin B1, B2, G1 and G2 were 0.04μg/kg, 0.01μg/kg, 0.20μg/kg and 0.02μg/kg respectively, the recoveries were from 81.0% to 115.0%, the relative standard deviations were less than 8.2%.

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Periodical:

Advanced Materials Research (Volume 1022)

Pages:

76-80

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1022.76

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Online since:

August 2014

Authors:

Yong Gang Shi*, Wei Zhong, Hao Wen, Ju Ma, Ping Sun

Keywords:

Aflatoxin, Derivation, Fluorescence, HPLC, Peanut

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* - Corresponding Author

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