Electrochemical Determination of Heparin in Pharmaceuticals with Using Malachite Green

Abstract:

In this paper for determination of heparin in pharmaceuticals by constant-current sweep voltammetry on the mercury-film electrode (MFE) we used cationic malachite green (MG) dye which is capable to form a complex with heparin. In the 0.05 M potassium tetraoxalate (KH₃C₄O₈ ∙ 2H₂O) buffer solution with pH 1.65 MG had a well-defined peak of electroreduction at -0.46 V (vs. CSE). During the study of electrochemical properties of heparin in complex with MG in cathodic potential range from 0.0 V to -1.3 V on MFE besides the signal from the dye at E = - 0.46 V the second signal at E = –0.87 V was found. Increasing the concentration of heparin in the solution led to decrease of signal intensity at E = -0.87 V. Optimum conditions for the electrochemical determination of heparin in dosage form with using MG were picked up. Influence of auxiliary components of heparin pharmacological solution on electroreduction of the complex MG-Hep was investigated. Under optimal conditions the decrease of reduction peak currents at E = -0.87 V was proportional to heparin concentration in the range from 3.9 ∙ 10^-4 mg/ml to 3.9 ∙ 10^-3 mg/ml.

• Periodical: Advanced Materials Research (Volume 1040)
• Pages: 292-296
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1040.292
• Online since: September 2014
• Authors: Darya A. Vishenkova*, E.I. Korotkova, Elena V. Dorozhko
• Keywords: Electroreduction, Heparin, Malachite Green, Mercury-Film Electrode, Voltammetry

References

[1] Haiying Liu, Zhenqing Zhang, Robert J. Linhardt, Lessons learned from the contamination of heparin, NPR, 26 (2009) 305-452.

[2] A.N. Bashkatov, E.A. Genina, V.V. Tuchin, Investigation of optical and diffusion phenomena in biotissues at the influence of osmotic active immersion liquids, Common biophysical practical work, Saratov State University, Saratov (2005).

DOI: 10.18500/1817-3020-2008-8-1-15-20

[3] V. A. Isaev, M. E. Grigoreva, L. A. Lyapina, L. S. Nicolaeva, A. N. Semenov, A. A. Ershov, V. N. Sergeev, Russian Patent 2454237 (2011) (In Russian).

[4] V. Yu. Khokhlov, V. F. Selemenev, O. N. Khohlova, R. L. Shcherbinin, Pharmaceutical Chemistry Journal, 33 (1999) 450-451.

[5] J. Manzoori, A. Jouyban, M. Amjadi, A. Ramezani, Journal of Applied Spectroscopy, 80 (2013) 104-110.

[6] Rahul P. Patel, Christian Narcowicz, Joseph P. Hutchinson, Emily F. Hilder, Glenn A. Jacobson, J. of Pharmaceutical and Biomedical Analysis, 46 (2008) 30-35.

[7] Sun Wei, Ding Yaqin, Jiao Kui., J. of Analytical Chem. 61 (2006) 359-364.

[8] Information on http: /www. sepax-tech. com.

[9] Zhou X.M., Liu J.W., Zhang M.E., Chen S.J.  Talanta, 46 (1998) 757-760.

[10] A. L. Bekrovsky, E. V. Sergeeva, A. V. Suvorov, A. A. Kozlov, RENAM, Moscow, 2011 p.15. (In Russian).

[11] Yu.M. Evdokimov, S.G. Skuridin, V.I. Salyanov, Russian 2440575 (2010). (In Russian).

[12] Z. Gauptman, Yu. Grefe, H. Pemane, Organic chemistry, Chemistry, Moscow, 1979. (In Russian).