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MAE-HPLC Determination of Bioactive Compounds in Gardenia jasminoides Ellis

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Abstract:

In this study, a novel microwave-assisted extraction (MAE) method has been developed for the extraction and determination of the major biologically active compounds, crocin and its seven trans-cis analogues in a traditional Chinese medicine (TCM), gardenia fruits (Gardenia jasminoides Ellis) with analysis by reversed phase HPLC. Sudan I [1-phenylazo-2-naphthol] was selected as the internal standard. The results of quantitative determination of seven crocin analogues in six commercial gardenia fruits samples show that MAE–HPLC is a simple, rapid, low cost and reliable method for the determination of biologically active compounds, crocin analogues in TCM, and also a potential and powerful tool for quality assessment of gardenia fruits (Gardenia jasminoides Ellis).

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Periodical:

Advanced Materials Research (Volumes 1073-1076)

Pages:

122-126

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1073-1076.122

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Online since:

December 2014

Authors:

Qiao Yang*

Keywords:

Crocin Analogue, Gardenia jasminoides Ellis, HPLC, Microwave-Assisted Extraction

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© 2015 Trans Tech Publications Ltd. All Rights Reserved

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* - Corresponding Author

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