Optimization and Modelling of Denitrification and Methanogenic Activity in Start up of Mixotrophic Anammox Reactor

Abstract:

In the present study, the SDA (specific denitrification activity) and SMA (specific methanogenic activity) in Start up of mixotrophic anammox reactor was optimized by applying the response surface method (RSM). The purpose of this work was to find the optimal combination of C/N ratio, influent ammonium (NH₄⁺-N) and volatile suspended solid (VSS) with respect to minmum the SDA and SMA. Based on the RSM results, the quadratic model developed for the responses indicated that optimal conditions were C/N ratio of 0.5, influent NH₄⁺-N content of 200mg L^-1, and VSS content of 59.31g L^-1. Under this conditions, the SDA and SMA were minimize and found to be 0.05 mmol N₂ (g VSS d)^-1, 0.017 mmol N₂ (g VSS d)^-1, respectively

• Periodical: Advanced Materials Research (Volumes 1073-1076)
• Pages: 127-135
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1073-1076.127
• Online since: December 2014
• Authors: Dun Tao Shu, Yan Ling He*, Qing Yi Wang, Wang Li
• Keywords: Box-Behnken Model, Denitrification Activity, Methanogenic Activity, Response Surface Methodology (RSM)

References

[1] G. Zhu, S. Wang, W. Wang, Y. Wang, L. Zhou, B. Jiang, et al. Hotspots of anaerobic ammonium oxidation at land-freshwater interfaces. Nature Geoscience, vol. 6(2013), pp.103-107.

DOI: 10.1038/ngeo1683

[2] A. A. Van de Graaf, P. de Bruijn, L. A. Robertson, M. S. Jetten, and J. G. Kuenen. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor. Microbiology, vol. 142(1996), pp.2187-2196.

DOI: 10.1099/13500872-142-8-2187

[3] M. Jetten, I. Cirpus, B. Kartal, L. van Niftrik, K. Van De Pas-Schoonen, O. Sliekers, et al. 1994–2004: 10 years of research on the anaerobic oxidation of ammonium. Biochem Soc Trans, vol. 33(2005), pp.119-123.

DOI: 10.1042/bst0330119

[4] J. G. Kuenen. Anammox bacteria: from discovery to application. Nature Reviews Microbiology, vol. 6(2008), pp.320-326.

DOI: 10.1038/nrmicro1857

[5] M. K. H. Winkler, J. Yang, R. Kleerebezem, E. Plaza, J. Trela, B. Hultman, et al. Nitrate reduction by organotrophic Anammox bacteria in a nitritation/anammox granular sludge and a moving bed biofilm reactor. Bioresource Technology, vol. 114(2012).

DOI: 10.1016/j.biortech.2012.03.070

[6] S. A. Maruyama, S. V. Palombini, T. Claus, F. Carbonera, P. F. Montanher, N. E. d. Souza, et al. Application of box-behnken design to the study of fatty acids and antioxidant activity from enriched white bread. Journal of the Brazilian Chemical Society, vol. 24(2013).

DOI: 10.5935/0103-5053.20130193

[7] M. Jain, V. Garg, and K. Kadirvelu. Investigation of Cr (VI) adsorption onto chemically treated Helianthus annuus,: Optimization using Response Surface Methodology. Bioresource technology, vol. 102(2011), pp.600-605.

DOI: 10.1016/j.biortech.2010.08.001

[8] K. Papadopoulou, I. M. Kalagona, A. Philippoussis, and F. Rigas. Optimization of fungal decolorization of azo and anthraquinone dyes via Box-Behnken design. International Biodeterioration & Biodegradation, vol. 77(2013), pp.31-38.

DOI: 10.1016/j.ibiod.2012.10.008

[9] H. Ebrahimzadeh, M. Behbahani, Y. Yamini, L. Adlnasab, and A. A. Asgharinezhad. Optimization of Cu (II)-ion imprinted nanoparticles for trace monitoring of copper in water and fish samples using a Box–Behnken design. Reactive and Functional Polymers, vol. 73(2013).

DOI: 10.1016/j.reactfunctpolym.2012.10.006

[10] C. Trigo, J. Campos, J. Garrido, and R. Mendez. Start-up of the Anammox process in a membrane bioreactor. Journal of Biotechnology, vol. 126(2006), pp.475-487.

DOI: 10.1016/j.jbiotec.2006.05.008

[11] K. -W. Jung, M. -J. Hwang, M. -J. Cha, and K. -H. Ahn. Application and optimization of electric field-assisted ultrasonication for disintegration of waste activated sludge using response surface methodology with a Box-Behnken design. Ultrasonics sonochemistry, (2014).

DOI: 10.1016/j.ultsonch.2014.06.012

[12] R. -C. Jin, B. -S. Xing, and W. -M. Ni. Optimization of partial nitritation in a continuous flow internal loop airlift reactor. Bioresource Technology, vol. 147(2013), pp.516-524.

DOI: 10.1016/j.biortech.2013.08.077

[13] R. -C. Jin, G. -F. Yang, J. -J. Yu, and P. Zheng. The inhibition of the Anammox process: A review. Chemical Engineering Journal, vol. 197(2012), pp.67-79.