Application of Carboxymethyl Chitosan on Adsorbent Detoxification of Paralytic Shellfish Poisoning Toxins from Chlamys nobilis

Abstract:

This study investigates the effectiveness of adding carboxymethyl chitosan during steam cooking as an adsorbent for detoxifying paralytic shellfish poisoning toxins (PSP toxins) in the scallop Chlamys nobilis. Toxin analysis using a mouse bioassay test and hydrophilic interaction liquid chromatography–tandem mass spectrometry method (LC–MS) showed that most of the PSP toxins (>80%) were contained in the visceral compartment of the raw scallops. Overall, 82.2Mu/100g of PSP toxins were released from scallop tissues during steaming. The toxicity of the soup steamed with 0.1%, 0.3%, 0.6%, 1.0% and 1.5% carboxymethyl chitosan decreased the toxin content by 35.6%, 44%, 48.4%, 53.3% and 56.9% (p<0.005), respectively. The relative PSP toxin content in the raw adductor muscle was comparable to those after steaming or steaming with carboxymethyl chitosan (p > 0.05).The PSP toxin concentrations in the adductor muscles, gills + mantle and visceral compartments steamed with 0.1%, 0.3%, 0.6%, 1% and 1.5% carboxymethyl chitosan were not significantly different from those in the corresponding raw samples. The LC–MS analysis showed that the adsorbance of carboxymethyl chitosan for each of the PSP toxins was positive correlation with concentration, although the uptake efficiency of each toxin was different. The reduction in toxin content of all analyzed toxins reached 45.6%, 30.2%, 44.5%, 55.9% and 37.6% under the corresponding carboxymethyl chitosan concentrationsis.

• Periodical: Advanced Materials Research (Volumes 1073-1076)
• Pages: 1798-1803
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1073-1076.1798
• Online since: December 2014
• Authors: Song Shan Zhang, Jing Jin, Qing Peng Li, Yi Ming Ha*
• Keywords: Adsorbent Detoxification, Carboxymethyl Chitosan, Chlamys Nobilis, LC-MS, Paralytic Shellfish Poisoning

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