Nanomagnetic Particle Anchored to Monodispers Porous Microspheres for Pectinase Immobilization

Abstract:

The idea of preparing the surface of chitosan layer pre-absorbed on the monodispers porous microsphere via layer by layer approach receives special relevance in enzyme technology. Moreover, the colloidal stability is not impeded by the adsorbed proteins despite the fact that up to 390.2 mg of enzyme adsorbed per gram of the carrier particles. Maximum relative activity was observed at 50 °C and pH 3.5. The activity half lives for native and bound states of enzyme were found as 14.27 d and 31.05 d, respectively. These particles, premodified with the layer of magnetic nanoparticles to impart the magnetic property, were repeatedly used as catalysts following their rapid and easy separation.

• Periodical: Advanced Materials Research (Volume 1088)
• Pages: 38-42
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1088.38
• Online since: February 2015
• Authors: Shu Xian Bi, Xiao Liang Zhang
• Keywords: Chitosan Immobilized Pectinase Enzyme, Layer by Layer, Magnetic Microsphere Porous

References

[1] Daniel N., Tran Kenneth J., Balkus Jr. ACS. Catal, 1 (2011) 956-960.

[2] Atieh Bahrami, Parisa Hejazi. J. Mol. Catal B: Enzym, 93 (2013) 1-7.

[3] S. Solís J., C. Martínez, J. Paniagua, M. Asomoza. J. Sol-Ge. l Sci. Technol, 37 (2006) 121-123.

[4] Yajun Wang, Frank Caruso. Chem. Mater, 17 (2005) 953-956.

[5] Hudson S., Cooney J., Magner E. Angew. Chem. Int. Ed, 47 (2008) 8582-8594.

DOI: 10.1002/anie.200705238

[6] Chen Z. J., Li X. H.,  Li C. Chin. J. Catal, 32 (2011) 155-159.

[7] Kouassi G K, Irudayaraj. J. Anal. Chem, 78 (2006) 3234-3237.

[8] Shik C T, Chih H Y, Xin G, Kin T. J. Phys. Chem. B, 110 (2006) 16914-16922.

[9] Kapish G, Asim K J, Sandeep Kumar, Mithu Maiti. J. Mol. Catal. B: Enzym, 98 (2013) 30-36.

[10] An Z H, Helmuth Mo¨hwald, Li J B. Biomacromolecules, 7 (2006) 580-583.

[11] Tong W J, Gao C Y, Helmuth Molhwald. Macromolecules, 39 (2006) 335-340.

[12] Ines Belhaj-Ben Romdhane, Zamen Ben Romdhane, Ali Gargouri, Hafedh Belghith. J. Mol. Catal. B: Enzym, 68 (2011) 230-239.

[13] Arpana Kumari, Arvind M. Kayastha. J. Mol. Catal. B: Enzym, 69 (2011) 8-14.

[14] Gong B L, Ke C Y, Geng X D. Chin. J. Chem, 22 (2004) 283-289.

[15] Sahoo Y, Goodarzi A, Swihart M T, Ohulchanskyy T Y, Kaur N, Furlani E P. J. Phy. Chem. B, 109 (2005) 3879-3885.

DOI: 10.1021/jp045402y

[16] Bailey M J, Pessa E. Enzyme. Microb. Technol, 12 (1990) 266-271.

[17] Miller G L. Ana. Che, 31(1959) 426-428.