A HPLC-UV Method for Simultaneous Determination of Hypocrellin A and B from Fermentation Broth of Shiraia Bambusicola LPHP-89

Sai Dan Zhang; Ping Lv; Xiu Zhu

doi:10.4028/www.scientific.net/AMR.1092-1093.662

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1092-1093A HPLC-UV Method for Simultaneous Determination of...

A HPLC-UV Method for Simultaneous Determination of Hypocrellin A and B from Fermentation Broth of Shiraia Bambusicola LPHP-89

Abstract:

A rapid, sensitive and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous quantification of hypocrellin A, hypocrellin B from Fermentation Broth of Shiraia bambusicola LPHP-89. Isocratic RP-HPLC system with C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) and a detector UV-VWD was employed. The mobile phase consisted of methanol、water and acetic acid (60:40:1, v/v/v) and was pumped at a flow rate of 1.0 mL/min. The injection volume was5 μL. The quantitation wavelength was set at 254 nm.Total run time was 20 min and retention times for hypocrellin A and hypocrellin B were 10.06 and 15.38 min, respectively.

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Periodical:

Advanced Materials Research (Volumes 1092-1093)

Pages:

662-665

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1092-1093.662

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Online since:

March 2015

Authors:

Sai Dan Zhang, Ping Lv*, Xiu Zhu

Keywords:

Fermentation, HPLC-UV, Hypocrellin A, Hypocrellin B, Shiraia Bambusicola

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