Inorganic-Organic Thin-Layers which Transform Bio-Molecule Binding into Visible Color Change

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SiO2 thin-films were prepared by thermal oxidation method. It showed uniform color based on the thin-film interference of visible light. Then we made biotin antenna on the top of SiO2 thin-film. Biotin is one of the vitamins and it has high association constant with protein avidin. After immersed the substrate into avidin solution, certain color changes based on the self-assembling of avidin on its surface were observed. By the immersing these substrates into mono-dispersed polystyrene particle solution, a polystyrene particle layer was formed onto avidin SAMs and it brought dynamic interference color change. As a result, this substrate could sense the avidin molecules over 1.0×10-8M of its concentration by naked eyes.

Info:

Periodical:

Advanced Materials Research (Volumes 11-12)

Main Theme:

Edited by:

Masayuki Nogami, Riguang Jin, Toshihiro Kasuga and Wantai Yang

Pages:

631-634

DOI:

10.4028/www.scientific.net/AMR.11-12.631

Citation:

R. Tominaga et al., "Inorganic-Organic Thin-Layers which Transform Bio-Molecule Binding into Visible Color Change", Advanced Materials Research, Vols. 11-12, pp. 631-634, 2006

Online since:

February 2006

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Price:

$35.00

[1] Y. Takeoka and M. Watanabe: Langmuir Vol. 19 (2003), p.9104.

[2] A.G. Mayes, J. Blyth, R.B. Millington and C.R. Lowe: Anal. Chem. Vol. 74 (2002), p.3649.

[3] Z. Gu, R. Horie, S. Kubo, Y. Yamada, A. Fujishima and O. Sato: Angew. Chem. Int. Ed. Vol. 41(7) (2002), p.1145.

[4] C.E. Reese, M.E. Baltusavich, J.P. Keim and S.A. Asher: Anal. Chem. Vol. 73 (2001), p.5038.

[5] A.G. Mayes, J. Blyth, M. Kyröläinen-Reay, R.B. Millington and C.R. Lowe: Anal. Chem. Vol. 71 (1999), p.3390.

DOI: 10.1021/ac990045m

[6] J.H. Holts, J.S.W. Holts, C.H. Munro and S.A. Asher: Anal. Chem. Vol. 70 (1998), p.780.

[7] R. Tominaga, T. Kobayashi, M. Sivakumar and T. Kinoshita: Trans. MRS-J Vol. 30 (2005), p.349.

[8] M. Sivakumar, R. Tominaga, T. Koga, T. Kinoshita, M. Sugiyama and K. Yamaguchi: Sci. Tech. Adv. Mater. Vol. 6 (2005), p.91.

[9] B. Steiger, C. Padeste, A. Grubelnik and L. Tiefenauer: Electrochimica Acta Vol. 48 (2003), p.761.

DOI: 10.1016/s0013-4686(02)00746-6

[10] N.M. Green: Biochem. J. Vol. 89 (1963), p.585.

[11] N.M. Green: Biochem. J. Vol. 89 (1963).

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