Cytotoxicity Assessment of SH-SY5Y Cells Grown on Graphene Sheet

Abstract:

We investigate the effect of cell culture conditions, using pristine graphene sheets as growth substrate, on the human nerve cell line (SH-SY5Y). In order to evaluate cell viability and morphology, we applied the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, and fluorescence microscopy of cells stained with Hochest 33342 and Calcein AM. Human nerve cells exhibited 84% viability on pristine graphene sheets compared with control (cell culture polystyrene) after 3 days culturing. Fluorescence data showed that the presence of graphene did not influence cell morphology. These results suggest that graphene sheets may be used for biological applications.

• Periodical: Advanced Materials Research (Volume 1110)
• Pages: 311-314
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1110.311
• Online since: June 2015
• Authors: Hong Gi Oh, Hyo Geun Nam, Kwang Hwan Jhee, Joon Mook Lim, Kwang Soup Song*
• Keywords: Cell Viability, Fluorescence, Graphene, MTT Assay, SH-SY5Y

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