Enzymatic Hydrolysis of Lignocellulosic Oil Palm Empty Fruit Bunch (EFB)

Abstract:

A preliminary study was performed on enzymatic hydrolysis process for treating empty fruit bunch (EFB) fibre. The bioconversion of cellulose hydrolysis was carried out with soluble cellulase from Trichoderma reesei as the biocatalyst. Crucial trends such as substrate and enzyme loading influencing the enzymatic reaction were also studied in order to enhance the cellulose conversion. The results indicate that as the enzyme loading was increased, the EFB conversion also increased until it reached 115.63 FPU/g of enzyme concentration, beyond this values, the reverse occurred. On the other hand, as the substrate loading was increased the conversion decreased. Inhibition of enzyme adsorption by hydrolysis products appear to be the main cause of the decreasing conversion at increasing enzyme loading and substrate loading.

• Periodical: Advanced Materials Research (Volume 1113)
• Pages: 305-310
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1113.305
• Online since: July 2015
• Authors: Qadly Ameen Pahlawi, Nazlee Faisal Ghazali*, Khairilanuar Mohd Hanim, Nik Azmi Nik Mahmood
• Keywords: Cellulase, Hydrolysis, Lignocellulose, Product Inhibition

References

[1] F. Hamzah, A. Idris, T.K. Shuan, Preliminary study on enzymatic hydrolysis of treated oil palm (Elaies) empty fruit bunch fibre (EFB) by using combination of cellulose and β 1-4 glucosidase. Biomass and Bioengineering. 35 (2011) 1055-1059.

DOI: 10.1016/j.biombioe.2010.11.020

[2] Y. Sun, J. Cheng, Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol. 83 (2002). 1-11.

[3] M. Galbe, G. Zacchi, A review of the production of ethanol from softwood. Appl Microbiol Biotechnol. 59 (2002) 618-28.

DOI: 10.1007/s00253-002-1058-9

[4] M. Mandels, E.T. Reese, Inhibition of cellulases and β-glucosidases. In: Reese ET (ed) Advances in enzymatic hydrolysis of cellulose and related materials. Pergamon, London. (1963) p.115–157.

DOI: 10.1016/b978-0-08-009947-7.50011-6

[5] A. Chesson, The maceration of linen flax under anaerobic conditions. J. Appl. Bacteriol. (1978) 45: 219.

DOI: 10.1111/j.1365-2672.1978.tb04217.x

[6] T.K. Ghose, Measurement of cellulase activities. Pure Appl Chem. (1987) 59: 257-68.

[7] A.A. Astimar, M. Husin, M. Anis, Preparation of cellulose from oil palm empty fruit bunches via ethanol digestion: effect of acid and alkali catalysts. J Oil Palm Res. 14 (2002) 9-14.

[8] H. Ma, W.W. Liu, X. Chen, X.J. Wu, Z.L. Yu, Enhanced enzymatic saccharification of rice straw by microwave pretreatment. Bioresour Technol. (2009) 100: 1279-84.

DOI: 10.1016/j.biortech.2008.08.045

[9] T. Rezanka, K. Sigler, Biologically active compounds of semi-metals. ChemInform. 39 (2008) 585-606.

DOI: 10.1002/chin.200822264

[10] F. Carrillo, M.J. Lis, X. Colom, M. Lo ́ pez-Mesas and J. Valldeperas. Effect of alkali pretreatment on cellulase hydrolysis of wheat straw: Kinetic study. (2005) Process Biochem. 40: 3360 – 3364.

DOI: 10.1016/j.procbio.2005.03.003

[11] C. Martin, G. J. d. M. Rocha, , J. R. A. d. Santos, , M. C. d. Albuqurque and, E.R. Gouveia Enzyme Loading Dependence of Cellulose Hydrolysis of sugarcane bagasse (2012) Quim. Nova. 35 (10) 1927 – (1930).

DOI: 10.1590/s0100-40422012001000007

[12] L. Rosgard, P. Andric, K. Dam-Johansen, S. Pedersen and A.S. Meyer. Effects of substrate loading on enzymatic hydrolysis and viscosity of pre-treated barley straw. (2007) Appl. Biochem. Biotechnol. 143 (1); 27 – 40.

DOI: 10.1007/s12010-007-0028-1

[13] Y. Zhao, B. Wu, B. Yan, P. Gao, Mechanism of cellobiose inhibition in cellulose hydrolysis by cellobiohydrolase. Sci Chin Ser C Life Sci. 47 (2004) 18–24.

DOI: 10.1360/02yc0163

[14] T.S. Khaw, and A.B. Ariff (2009) Optimization of enzymatic saccharification of palm oil mill effluent solid and oil palm fruit fiber to fermentable sugars. J. Trop. Agric. And Fd. Sci. 37 (1): Page 85- 94.

DOI: 10.1016/j.biortech.2020.123723

[15] V. Rani, S. Mohanram, R. Tiwari, L. Nain, and A. Arora (2014) J. Bioprocess. Biotech. 5: 197 doi: 10. 4172/2155-9821. 1000197.

[16] S. Shahbazi, H. Askari, T. Naseripour, M. Moosavi-Nasab and, M. Bakhtiyari (2014).

[17] Y. C. Ching and T. S. Ng (2014) Effect of Preparation Conditions on Cellulose from Oil Palm Empty Fruit Bunch Fiber. Bioresources. 9 (4), 6373 – 6385.

DOI: 10.15376/biores.9.4.6373-6385