Optical Trapping and Manipulation of Polymer Spheres and HeLa Cell Organelles

Anna Statsenko; Ginga Ito; Wataru Inami; Yoshimasa Kawata; Leonid Poperenko

doi:10.4028/www.scientific.net/AMR.1117.60

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Piezoresistivity and Electrical Resistance Relaxation of Polyisoprene Nanostructured Carbon Allotrope Hybrid Composites
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Optical Trapping and Manipulation of Polymer Spheres and HeLa Cell Organelles
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1117Optical Trapping and Manipulation of Polymer...

Optical Trapping and Manipulation of Polymer Spheres and HeLa Cell Organelles

Abstract:

Two types of single beam laser traps have been built. Laser trap using visible light is used to optically trap micro-and nanosized polymer spheres. Laser trap using near infrared radiation is used to avoid optical damage when used to manipulate living cells. Manipulation of internal organelle was successfully demonstrated.

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Periodical:

Advanced Materials Research (Volume 1117)

Pages:

60-64

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1117.60

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Online since:

July 2015

Authors:

Anna Statsenko*, Ginga Ito, Wataru Inami, Yoshimasa Kawata, Leonid Poperenko

Keywords:

Hela Cell, Laser Trap, Optical Manipulation

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* - Corresponding Author

References

[1] Xie, C. G., Dinno, M. A. & Li, Y. Q. Near-infrared Raman spectroscopy of single optically trapped biological cells. Opt. Lett. 27, 249–251 (2002).

DOI: 10.1364/ol.27.000249

Google Scholar

[2] Zheng, F., Qin, Y. & Chen, K. Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer. J. Biomed. Opt. 12, 034 002 (2007).

DOI: 10.1117/1.2748060

Google Scholar

[3] Williams, M. C., Rouzina, I., Wenner, J. R., Gorelick, R. J., Musier-Forsyth, K. & Bloomﬁeld, V. A. 2001 Mechanism for nucleic acid chaperone activity of HIV-1 nucleocapsid single bacterial cells in aqueous solution using conﬂocal laser tweezers Raman spectroscopy. Anal. Chem. 77, 4390–4397.

DOI: 10.1073/pnas.101033198

Google Scholar

[4] Huebsch, N. D. & Mooney, D. J., Fluorescent resonance energy transfer: a tool for probing molecular cell–biomaterial interactions in three dimensions. Biomaterials 28, 2424–2437 (2007).

DOI: 10.1016/j.biomaterials.2007.01.023

Google Scholar

[5] Lee, S. H. & Grier, D. G. Holographic microscopy of holographically trapped three-dimensional structures. Opt. Express 15, 1505–1512 (2007).

DOI: 10.1364/oe.15.001505

Google Scholar

[6] GoksЁor, M., Enger, J. & Hanstorp, D/ Optical manipulation in combination with multiphoton microscopy for single-cell studies. Appl. Opt. 43, 4831–4837.

DOI: 10.1364/ao.43.004831

Google Scholar

[7] Laser induced cell fusion in combination with optical tweezers: the laser cell fusion trap. Cytometry 12, 505–510.

DOI: 10.1002/cyto.990120607

Google Scholar

[8] Visscher, K., Brakenhoff, G. J. & Krol, J. J. 1993 Micro-manipulation by multiple optical traps created by a single fast scanning trap integrated with the bilateral confocal scanning laser microscope. Cytometry 14, 105–114. (doi: 10. 1002/cyto. 990140202).

DOI: 10.1002/cyto.990140202

Google Scholar

[9] Steabing R. W et al., Cytometry 12, pp.505-510, (1991).

Google Scholar

[10] Joel Voldman, Dielectrophoretic Traps for Cell Manipulation, BioMEMS and Biomedical Nanotechnology 2007, pp.159-186.

DOI: 10.1007/978-0-387-25845-4_8

Google Scholar