Cloning of Salt-Resistance Transcription Factor Gene and Identification of Function in Soybean Plants
In order to verify the transcription factor CBF2 gene can be induced to express in plant and improve the plant salt resistance, they were cloned from the Arabidopsis thaliana by PCR according to CBF2 gene sequence published on GenBank. The plant expression vector (pBI-rd29A-CBF2) with CBF2 gene was constructed. The CBF2 gene was transformed into soybean plants via Agrobacterium-mediated transformation. It approved that the CBF2 gene had been transformed and integrated into the genome of soybean by PCR and Southern blot. Through the physiological test of salt resistance, the relative electric conductivity of the transformed plants plasma membrane and the control were 36.84% and 59.35% respectively under salt stress. The content of chlorophyll of the transformed plants was 2.41 times as much as that of the control. The content of proline of the transformed plants was 2.49 times as much as that of the control. It showed that the CBF2 gene was induced to express in soybean plants. And it also indicated that the expression of the CBF2 gene increased the salt stress tolerance of the transgenic soybean plants. The results proved the CBF2 gene were functional gene.
Zhenyu Du and X.B Sun
M. Qu et al., "Cloning of Salt-Resistance Transcription Factor Gene and Identification of Function in Soybean Plants", Advanced Materials Research, Vols. 113-116, pp. 1324-1327, 2010