The filter media from iron and manganese biological removal filter which w- as replaced by new termly can be re-used. The sand filter media was characterized u- sing the SEM and owns developed interspaces. Drying the sand filter media at 110°C, then inspect and analyse its character and mode of defluoridation。The results show as follows. Under the test condition ,the adsorption balance can come out in about 3 hou-rs , the fluoride sorption on the re-used matured filter media followed pseudo-second kinetics. The effect of defluoridation by the matured filter media will be better within the range of pH 3~7, and best at about pH 4.58. The primary mode of fluoride sorpti- on is chemism, but physical sorption is also in existence , and the adsorption isother- ms could be described by Langmuir equation. Contact-time is a vary important factor in the continuous-flow column experiment. The effect of fluoride removal is long，as the height of filter column is controlled as 40cm.The filter media from iron and manga- nese biological removal filter possess potential of perfect sorbent, space for deeply study and foreground of practical application.