Purification and Characterization of Laccase from Curvularia trifol

Abstract:

Electrophoretic pure lacquer enzymes were obtained from the thick enzyme of Gladiolus Curvularia trifol with grading precipitation and DEAE2-Cellulose ion exchange chromatography. The overall recycling rate of enzyme activity was 14.97% and the purification reached 2.96 times. The relative molecular mass of enzyme was 92.3Kda. The optimal temperature and pH were 40°C and 3.5, respectively. The Km of ABTS catalyzed by laccase was 1.11× 10- 5mol L-1. Alizarin red and Congo red could be degradated by purified laccase effectively without the participation of small molecule amboceptor. Alizarin red could be degradated by 80% with its being affected by 1000U/L of enzyme activity for 70h, which revealed the huge potentiality of Gladiolus Curvularia trifol and laccase in the degradation of dye.

• Periodical: Advanced Materials Research (Volumes 113-116)
• Pages: 2215-2219
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.113-116.2215
• Online since: June 2010
• Authors: Jie Zhang, Jing Ren, Yang Yu, Bin Song Wang
• Keywords: Curvularia trifol, Enzymological Properties, Laccase, Purification of Enzyme

References

[1] Thurston C. F.: The structure and function of fungal laccase. Microbiology, 19-26 , (1994), p.114.

[2] Xiaoshuang Song, Minzhao: North shuttle capsule Kong Laccase Gene Cloning and DNA sequence analysis. Journal of Beijing Forestry University, 59-64 , (2005), p.27.

[3] Lei Lu, Min Zhao, Bei-Bei Zhang, et al.: Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme. Appl Microbiol Biotechnol, 1232-1239 , (2007), p.74.

DOI: 10.1007/s00253-006-0767-x

[4] Murugesan K., Arulmani M., In-Hyun Nam, et al.: Purification and characterization of laccase produced by a white rot fungus Pleurotus sajor-caju under submerged culture condition and its potential in decolorization of azo dyes. Appl Microbiol Biotechnol, 939-946, (2006).

DOI: 10.1007/s00253-006-0403-9

[5] Rao Jun Guo: Protein electroPHoresis experimental technique. Bei jing: Science Press, 53-66 , (2005).

[6] Madhavi S, Revankar S S, Lele: Synthetic dye decolorization by white rot fungus, Ganoderma s pWR-1. Bioresource Technology, 775-780, (2007).

DOI: 10.1016/j.biortech.2006.03.020

[7] Murugesan K, N am IH, Kim YM , etal. : Decolorization of reactive dyes by a thermostable laccase p roduced by Ganodem a lucidum in solid state culture . Enzyme and M icrobial Technology, 1662-1672 , (2007).

DOI: 10.1016/j.enzmictec.2006.08.028