Dyp-Type Peroxidase (DypA) from the Bioleaching Acidophilic Bacterium Leptospirillum ferriphilum DSM 14647

Mackarena Contreras; Carolina Mascayano; Renato Chávez; Alonso Ferrer; Barulio Paillavil; Gloria J. Levicán

doi:10.4028/www.scientific.net/AMR.1130.23

Paper Titles

Microbial Communities in Sediments in Acidic, Metal-Rich Mine Lakes: Results from a Study in South-West Spain
p.7

Influence of Different Growth Conditions on the Composition of Extracellular Polymeric Substances of Acidithiobacillus ferrooxidans and Acidithiobacillus ferrivorans Species
p.11

Comparative Genomics Underlines the Functional and Taxonomic Diversity of Novel “Ferrovum” Related Iron Oxidizing Bacteria
p.15

Screening and Isolation of Bacterial Strains with the Biotechnological Potential to Destruct Cyanide-Bearing Compounds under the Conditions of Extreme Continental Climate
p.19

Dyp-Type Peroxidase (DypA) from the Bioleaching Acidophilic Bacterium Leptospirillum ferriphilum DSM 14647
p.23

Global Transcriptional Responses of Acidithiobacillus ferrooxidans Wenelen under Different Sulfide Minerals
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Comparison of Microbial and Geochemical Conditions of Lignite Coal Spoil and Overburden Areas and their Environmental Impact
p.32

Characteristics of Acidibacillus Spp.: A Novel Genus of Acidophilic Iron-Oxidising Firmicutes
p.36

Microarray-Based Characterization of Microbial Community Functional Structure and Heterogeneity Associated with Acid Mine Drainages
p.40

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1130Dyp-Type Peroxidase (DypA) from the Bioleaching...

Dyp-Type Peroxidase (DypA) from the Bioleaching Acidophilic Bacterium Leptospirillum ferriphilum DSM 14647

Abstract:

Leptospirillum ferriphilum is an acidophilic iron-oxidizing bacterium that is relevant for chemical leaching of sulfide ores. In the extremely acidic conditions found in bioleaching operations, this microorganism deals with an abundant supply of soluble iron and other metals that might induce oxidative damage to biomolecules through the generation of reactive oxygen species (ROS). We evaluated the role of Dyp-type peroxidase in the protection against oxidative stress in L. ferriphilum DSM14647. The genetic region encoding dypA was cloned and sequenced. The predicted DypA enzyme is 295 amino acids long with an estimated molecular mass of 32.9 kDa containing a highly conserved peroxide reduction motif. Genetic complementation of catalases/peroxidases-deficient Escherichia coli cells indicated that expression of dypA from L. ferriphilum restored the resistance to hydrogen proxide to levels exhibited by the wild type strain. Exposure of L. ferriphilum to hydrogen peroxide leads to a significant transcriptional activation of dypA suggesting its involvement in the response to oxidative stress in this bacterium. This is the first Dyp-type peroxidase characterized from an acidophilic microorganism, making it a potential candidate for research in basic and applied biology.

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Periodical:

Advanced Materials Research (Volume 1130)

Pages:

23-27

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1130.23

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Online since:

November 2015

Authors:

Mackarena Contreras, Carolina Mascayano, Renato Chávez, Alonso Ferrer, Barulio Paillavil, Gloria J. Levicán*

Keywords:

Bioleaching, Dyp-Type Peroxidase, Leptospirillum Ferriphilum, Oxidative Stress

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