Detection of Antibiotic-Resistant Klebsiella Sp. from the Wupa River and Wastewater Treatment Plant, F.C.T Abuja

Abstract:

Antimicrobial resistance (AMR) is a global health crisis that undermines the effectiveness of antibiotics in treating infections, posing a threat to humans and animals. Antibiotics are not fully metabolized by humans or animals, leading to their dispersion within sewage streams. The dissemination of antibiotic-resistant bacteria in aquatic environments poses a significant public health risk, particularly through the release of treated wastewater into natural water bodies. This study investigated the presence of Antibiotic Resistance Genes (ARGs) in Klebsiella sp. isolates collected from four sampling points along the Wupa River and its wastewater treatment plant (WWTP): Upstream (P1), Influent (P2), Effluent (P3), and Downstream (P4). Physicochemical analyses of water samples showed that most parameters, including temperature, pH, total dissolved solids, dissolved oxygen, and nitrates, stayed within WHO-recommended limits. However, phosphates, total suspended solids, and ammoniacal nitrogen consistently exceeded these limits at all sites. The highest levels of biochemical oxygen demand (BOD), soluble chemical oxygen demand (COD), and total COD were found at the influent. The Total Viable Count carried out for bacterial colonies and Klebsiella colonies to assess microbial load showed the highest value at the 10^ (- 2) dilution factor and the lowest at the 10^ (- 5) dilution factor. Isolation and identification of Klebsiella spp. were performed using biochemical tests, including Voges-Proskauer (+), Indole (-), Citrate (+), Urease (+), Methyl red (-), Triple Sugar Agar (+), and Gram staining, revealing Gram-negative rods. Molecular analysis detected amplification of key resistance genes sul2, tetB, blaTEM, and blaCTX. Amplification of tetB gene in both influent and effluent samples indicates incomplete removal of tetracycline resistance genes during wastewater treatment. The blaTEM and blaCTX genes were detected across all sampling points, suggesting widespread environmental dissemination and potential contamination sources beyond the WWTP. These findings highlight the persistence and spread of multidrug-resistant Klebsiella in riverine and wastewater environments, underscoring the critical need for improved wastewater treatment strategies and continuous monitoring to mitigate the public health impact of antibiotic resistance in aquatic ecosystems.

• Periodical: Advanced Materials Research (Volume 1189)
• Pages: 121-132
• DOI: https://doi.org/10.4028/p-BD62ux
• Online since: March 2026
• Authors: Rabi Ahmed*, George Mangse, Azeez Olalekan Omotosho, Umar Farouk, Winifred Ofure Brown, Zarah Ismail Musa
• Keywords: Antibiotic Resistance Genes (ARGs), Klebsiella Sp., Wastewater, Wupa River

References

[1] Adegoke, A. A., Madu, C. E., Aiyegoro, O. A., & Okoh, A. I. (2020). Antibiotic resistance in bacteria from wastewater treatment plants in Nigeria. Journal of Environmental Science and Health, Part A, 55(1), 1–10

[2] World health Organization (WHO 2023). Global antimicrobial resistance and use surveillance system (‎GLASS)‎ report: 2022:  Global report

DOI: 10.2471/b09585

[3] Cheng, D., Feng, Y., Liu, Y., Xue, J., and Li, Z. (2019). Dynamics of oxytetracycline, sulfamerazine, and ciprofloxacin and related antibiotic resistance genes during swine manure composting. J. Environ. Manag. 230, 102–109

DOI: 10.1016/j.jenvman.2018.09.074

[4] Ghebremedhin, B., Olugbosi, M. O., Raji, A. M., Layer, F., Bakare, R. A., Konig, B., & Konig, W. (2009). Emergence of a community-associated methicillin-resistant Staphylococcus aureus strain with a unique resistance profile in southwest Nigeria. Journal of Clinical Microbiology, 47(9), 2975–2980

DOI: 10.1128/JCM.00648-09

[5] Lamikanra, A., Crowe, J. L., Lijek, R. S., Odetoyin, D. W., Wain, J., Aboderin, A. O., & Okeke, I. N. (2011). Rapid evolution of fluoroquinolone-resistant Escherichia coli in Nigeria is temporarily associated with fluoroquinolone use. BMC Infectious Diseases, 11, 312

DOI: 10.1186/1471-2334-11-312

[6] Okesola, A. O., & Oni, A. A. (2009). Antimicrobial resistance among common bacterial pathogens in south western Nigeria. American-Eurasian Journal of Agricultural & Environmental Sciences, 5(3), 327–330.

[7] Melander, E., Jonsson, G., & Molstad, S. (2000). Frequency of penicillin-resistant pneumococci in children is correlated to community utilization of antibiotics. Pediatric Infectious Disease Journal, 19(12), 1172–1177

DOI: 10.1097/00006454-200012000-00011

[8] Mohammed, Y. M., Arimoro, F. O., Ayanwale, A. V., Adamu, K. M., Keke, U. N., Abubakar, M. D., & Achebe, A. C. (2021). The current state of water quality and benthic invertebrate fauna in Chikke Stream (North-Central Nigeria). Ukrainian Journal of Ecology, 11(3), 26–34

[9] U.F. Ibrahim, K.M. Adamu, S.S.D. Mohammed, M.N. Chukwu, and H.F. Umar, "Utilization of Bacillus subtilis and Aspergillus niger for the Bioremediation of Heavy Metals in the WUPA Wastewater Treatment Plant, Abuja" The 2nd International Conference on Multidisciplinary Engineering and Applied Sciences (ICMEAS-2023) 2023.

DOI: 10.1109/icmeas58693.2023.10379287

[10] Wang, J., Mao, D., Mu, Q., & Luo, Y. (2020). Fate and proliferation of typical antibiotic resistance genes in wastewater treatment plants: A review. Environemntal science and Pollution research, 45, 131-139

DOI: 10.1016/j.mib.2018.04.004

[11] Rizzo, L., Manaia, C., Merlin, C., Schwartz, T., Dagot, C., Ploy, M. C., ... & Fatta-Kassinos, D. (2021). Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: A review. Science of the Total Environment, 447, 345–360

DOI: 10.1016/j.scitotenv.2012.12.038

[12] Podschun, R., & Ullmann, U. (1998). Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical Microbiology Reviews, 11(4), 589–603.

DOI: 10.1128/cmr.11.4.589

[13] Bagley, S. T. (1985). Habitat association of Klebsiella species. Infection Control, 6(2), 52–58.

[14] Bouza, E., & Cercenado, E. (2002). Klebsiella and Enterobacter: Antibiotic resistance and treatment implications. Seminars in Respiratory Infections, 17(3), 215–230

DOI: 10.1053/srin.2002.34693

[15] Wyres, K. L., & Holt, K. E. (2018). Klebsiella pneumonia as a key trafficker of drug resistance genes from environmental to clinically important bacteria. Curremt opinions in Microbiology, 45, 131-139

DOI: 10.1016/j.mib.2018.04.004

[16] Parker, J. K., Gu, R., Estrera, G. A., Kirkpatrick, B., Rose, D. T., Mavridou, D. A., ... & Davies, B. W. (2023). Carbapenem-resistant and ESBL-producing Enterobacterales emerging in Central Texas. Infection and Drug Resistance, 16, 1249–1261

DOI: 10.2147/IDR.S403448

[17] Maishanu, F., Adamu, K. M., & Mohammed, Y. M. (2022). Anthropogenic impact on some water quality characteristics of Wupa River Federal Capital Territory, Abuja, Nigeria. Issues in Biological Sciences and Pharmaceutical Research, 10(3), 30–38. https://doi.org/10.15739/ ibspr.22.006

DOI: 10.15739/ibspr.22.006

[18] Mohammed, H., Adamu, K.M., Kasim, A.A., Mohammed, Y.M. & Dadi-Mamud, N.J. (2023). Bacteriological Analysis of Water from Emu-Stream, Kusotachin, Nigeria. Lapai Journal of Applied and Natural Sciences, 8(1): 38-44

[19] Cheesbrough, M. (2006). District laboratory practice in tropical countries second update (PART 2). Tropical Health Technology Publishers, Great Britain. 62-70pp

[20] Froböse, N. J., Bjedov, S., Schuler, F., Kahl, B. C., Kampmeier, S., & Schaumburg, F. (2020). Gram staining: A comparison of two automated systems and manual staining. Journal of Clinical Microbiology, 58, e01914-20

DOI: 10.1128/jcm.01914-20

[21] Akpen, G., Ekanem, E., & Agunwamba, J. (2016). The effects of sewage effluent discharges on the water quality of Wupa river in Abuja, Nigeria. Journal of Science and Technology (Ghana), 36(2), 73–85

DOI: 10.4314/just.v36i2.8

[22] Adesakin, T. A., Oyewale, A. T., Bayero, U., Mohammed, A. N., Aduwo, I. A., Ahmed, P. Z., Abubakar, N. D., & Barje, I. B. (2020). Assessment of bacteriological quality and physico-chemical parameters of domestic water sources in Samaru community, Zaria, Northwest Nigeria. Heliyon, 6(8), e04773

DOI: 10.1016/j.heliyon.2020.e04773

[23] Ali, H. H., Sridhar, B. B. M., & Rosenzweig, J. A. (2021). Klebsiella spp. isolates from Houston bayous exhibit increased resistance to lead exposure and possess enhanced virulence potential. Science of the Total Environment, 789, 147818

DOI: 10.1016/j.scitotenv.2021.147818

[24] Laht, M., Karkman, A., Voolaid, V., Ritz, C., Tenson, T., (2014). Abundances of tetracycline, sulphonamide and beta-lactam antibiotic resistance genes in conventional wastewater treatment plants (WWTPs) with different waste load. PLos OnE 9(8). e103705

DOI: 10.1371/journal.pone.0103705

[25] Dameanti FNAEP, Yanestria SM, Effendi MH, Plumeriastuti H, Tyasningsih W, Ugbo EN, Sutrisno R and Safri MAA (2025) Genotyping of extended spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia, Veterinary World, 18(1): 162–171

DOI: 10.14202/vetworld.2025.162-171

[26] A., Obasi, S. C. Nwachukwu, E. Ugoji, C. Kohler, A. Göhler, V. Balau, Y. Pfeifer, & I. Steinmetz. (2017). Extended-spectrum β-lactamase-producing Klebsiella pneumoniae from pharmaceutical wastewaters in South-Western Nigeria. Microbial Drug Resistance, 00(00)

DOI: 10.1089/mdr.2016.0269

[27] Obayiuwana, A., & Ibekwe, A. M. (2020). Antibiotic Resistance Genes Occurrence in Wastewaters from Selected Pharmaceutical Facilities in Nigeria. Water, 12(7), 1897

DOI: 10.3390/w12071897

[28] GBD 2021 Antimicrobial Resistance Collaborators (2024). Global burden of bacterial antimicrobial resistance 1990-2021: a systematic analysis with forecasts to 2050. Lancet (London, England), 404(10459), 1199–1226

DOI: 10.1016/S0140-6736(24)01867-1

[29] Kolte, B., & Nübel, U. (2024). Genetic determinants of resistance to antimicrobial therapeutics are rare in publicly available Clostridioides difficile genome sequences. The Journal of antimicrobial chemotherapy, 79(6), 1320–1328

DOI: 10.1093/jac/dkae101

[30] Suriyakhun, N., Jangsangthong, A., Tunyong, W., Kong-Ngoen, T., Santajit, S., Indrawattana, N., & Buranasinsup, S. (2024). Investigation of antimicrobial resistance and antimicrobial resistance genes in Staphylococcus aureus and coagulase-negative staphylococci isolated from rabbit. Veterinary world, 17(6), 1328–1335

DOI: 10.14202/vetworld.2024.1328-1335

[31] Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2020). PAST software: Paleontological statistics software package for education and data analysis (Version 4.02) [Computer software].

[32] Clinical and Laboratory Standards Institute, 2016. Performance Standards for Antimicrobial Susceptibility Testing. Clinical and Laboratory Standards Institute, Wayne, PA

DOI: 10.1201/9781420014495-6