Fabrication of a β-TCP Nanomaterial and its Inhibitory Effects on Human Ovarian Cancer SKOV-3 Cells

Abstract:

Nanosized β-tricalcium phosphate (TCP) material was produced in this study using a wet precipitation method and characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). Human ovarian sarcoma SKOV-3 cells were cultured and the influence of nanoscale β-TCP particles on SKOV-3 cell behavior was studied in vitro. As a result, β-TCP nanoparticles with average size of 100 nm were obtained. Cell growth of SKOV-3 cells was noticeably declined in the presence of β-TCP nanoparticles (200ng/ml). The distribution of cell cycle for SKOV-3 cells cultured with and without β-TCP nanomaterials was quite different. In G1 phase of cell cycle, the percentage of SKOV-3 cells cultured in the absence of β-TCP nanoparticles was significantly lower than that cultured in the presence of β-TCP nanoparticles (p<0.01). In S phase of cell cycle, on the other hand, the percentage of SKOV-3 cells cultured without β-TCP nanoparticles was noticeably increased compared with that cultured with β-TCP nanoparticles (p<0.01). Moreover, the expression of proliferating cell nuclear antigen (PCNA) in SKOV-3 cells cultured in medium containing 200ng/ml β-TCP nanopaticles was significantly lower than that in the cells cultured without β-TCP nanoparticles (p<0.01). In conclusion, the nanoscale β-TCP material synthesized in this study can exert anti-tumor effects on SKOV-3 cells through mechanisms of cell growth inhibition, downregulation of PCNA expression and cell cycle arrest at G1 phase.

• Periodical: Advanced Materials Research (Volumes 129-131)
• Pages: 1029-1033
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.129-131.1029
• Online since: August 2010
• Authors: Xiang Dong Ma, Xiao Ming Wu, Hai Xia Duan, Xing Ma, Tao Fu
• Keywords: Anti-Tumor Effects, Cancer, Cell Cycle Arrest, Nanomaterial, β-Tricalcium Phosphate (β-TCP)

References

[1] G.S. Shekhawat and V. Arya: Adv. Mater. Res Vol. 67 (2009), p.295.

[2] L. Bakhtiari, H.R. Rezaie, S.M. Hosseinalipour and M.A. Shokrgozar: J. Nano. Res Vol. 11 (2010), p.67.

[3] X. Ma, X. Wu, C. Wang, R. Lv and J. Wang: submitted to Chinese Biomaterials Congress (2010).

[4] X. Ma, Y.Y. Hu, X.M. Wu, J. Liu, Z. Xiong, Y.N. Yan, R. Lv and J. Wang: Mater. Sci. Forum Vol. 627-627 (2009), p.553.

[5] X. Ma, X. Wu, Y. Wu, J. Liu, Z. Xiong, R. Lv, Y. Yan, J. Wang and D. Li: J. Bioact. Comp. Polym Vol. 24 (2009), p.457.

[6] X. Ma, X. Wu, Y. Hu, Z. Xiong, R. Lv, J. Wang, D. Li and Y. Yan: J. Bioact. Comp. Polym Vol. 24 (2009), p.146.

[7] A.F. El-kott, M.A. El-baz and A.A. Mokhtar: Int. Urol. Nephrol Vol. 38 (2006), p.237.

[8] X. Ma and Q.P. Wang: J. Clin. Exp. Pathol Vol. 14 (1998), p.480.