The supramolecular interaction of curcumin and RM-β-CD-m was studied by spectrophotometry, and the apparent formation constant for curcumin-RM-β-CD-m complex was determined. The results showed that a 1:1 complex between curcumin and RM-β-CD-m was formed, and the apparent formation constants increase with the increase of reaction temperature. The thermodynamic parameters, standard free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) for complex formation of curcumin with RM-β-CD-m were calculated. ΔG° value indicates that the complex formation is a spontaneous, endothermic process, and the main driving force for complex formation is entropy. The inclusion complex was confirmed by infrared spectrum (IR).