the oxidation resistance of glass-based coating on molybdenum substrate was studied in the paper. The Mo substrates with glass based coating modified by the different concentration of ZrO2 in the coating were prepared and characterized; results showed the oxidation resistance of samples increased firstly and decreased subsequently with increase in ZrO2 concentrations. The influence of different heat treatment temperature was also discussed in the paper. After the sampes were pretreated at the high temperature for 20 minutes, the oxidation resistance properties were improved five times than un-pretreated ones. SEM showed the glass powders in the coating changed into viscous liquid which adhere to the substrate tightly after the samples had been pretreated. Resulting showed the sample exhibited the best oxidation resistance when pretreated at 1300°C.