The marked brittleness of concrete could be overcome by the addition of fibers. This paper experimentally investigated the mechanical properties and constitutive relationship of different fiber reinforced concrete. It is shown from the results that the compressive strength and peak strain of concrete with fiber have little improvement, but the ultimate strain, deformation capacity, toughness and energy dissipation capacity are improved greatly. The damage constitutive model recommended by the emendatory code for design of concrete structure (appendix C) (GB50010-2002) is applied for calculations and analyses according to the test results. The damage constitutive model and non-elastic constitutive model of different fiber reinforced concrete are established based on the test results. It is indicated from the analyses that the constitutive models established in this paper are in accordance with the characteristic of the fiber reinforced concrete in loading process. The damage constitutive model in appendix C in code could be applied directly in some low precision calculation and engineering application.