CY7C68013 data acquisition card generally connect GPIF with other peripherals through the external FIFO, not only increasing the device but getting poor real-time relatively. This paper, USB2.0-Based data acquisition system, solves this problem successfully. The system based on high-speed USB2.0 transmission control chip CY7C68013, uses GPIF connected A / D device directly. Calculate time intervals correctly, not only avoiding data loss, but also saving the FIFO memory and related connections, which greatly reduce costs. This paper describes how the GPIF connects to A / D chip as well as designing of firmware and application programs.