Sequence Length Variation of Internal Genic Space of 16S rDNA-23S rDNA in Bacterium with High Yield of Hydrogen Production

Yong Feng Li; Yi Xuan Wang; Lu Wang; Zhan Qing Wang

doi:10.4028/www.scientific.net/AMR.183-185.1413

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 183-185Sequence Length Variation of Internal Genic Space...

Sequence Length Variation of Internal Genic Space of 16S rDNA-23S rDNA in Bacterium with High Yield of Hydrogen Production

Abstract:

To develop the identification of species for fermentative biohydrogen-producing bacterium, scholars have found a method which is based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions. In the study, a large fragment of the rDNA operon, including the 16S rDNA, the intergenic spacer region (ISR) and approximately 2000 bases of the 23S rDNA, were polymerasechain reaction (PCR) amplified. The PCR amplification of the genomic DNA of Leptonema ilk strain 3055 using primers directed against conserved regions of the rRNA operon provided evidence that the 16S and 23S rRNA genes were linked via an intergenic spacer region. The sequencing of the intergenic spacer region indicated that it was 435 nucleotides in length and sequence similarity searches revealed that it bore no homology to any known sequences including tRNA available in databases.

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Periodical:

Advanced Materials Research (Volumes 183-185)

Pages:

1413-1416

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.183-185.1413

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Online since:

January 2011

Authors:

Yong Feng Li, Yi Xuan Wang, Lu Wang, Zhan Qing Wang

Keywords:

16S-23S rDNA Intergenic Region, Biohydrogen, H₂-Producing Bacterium, Length Variation

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