Study on the Biological Activity of Anti-Tumor Peptide of Tumstatin

Shu Jing Wang; Ning Chen; Zhang Yi; Jia Liu; Bei Bei Xu

doi:10.4028/www.scientific.net/AMR.183-185.1509

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 183-185Study on the Biological Activity of Anti-Tumor...

Study on the Biological Activity of Anti-Tumor Peptide of Tumstatin

Abstract:

Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma cell. To study its effect on the proliferation and apoptosis of different tumor cells and verify its anti-tumor non-specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT assay, cell growth curve, TUNEL assay，flow cytometry，transmission electron microscopy(TEM), the biological activity of 19peptide was studied. Experiments in vitro identified that obtained 19peptide could inhibit proliferation of hela cell and hepatocarcinoma cell. It also could promote two tumor cells apoptosis. Its anti-tumor effect will lay foundation on its mechanism of action research and clinically tumor therapy in future.

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Periodical:

Advanced Materials Research (Volumes 183-185)

Pages:

1509-1512

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.183-185.1509

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Online since:

January 2011

Authors:

Shu Jing Wang, Ning Chen, Zhang Yi, Jia Liu, Bei Bei Xu

Keywords:

Cell Apoptosis, Cell Proliferation, Protein Expression, Tumstatin

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