Gill ATPase Activities and Microstructures of Juvenile Chinese Mitten Crab, Eriocheir sinensis, Exposed to Ambient Mercury
Experiments were carried out to investigate the effects of ambient mercury (Hg2+) on gill Na+-K+-ATPase, Ca2+-Mg2+-ATPase activities, and gill microstructure of juvenile Chinese mitten crab, Eriocheir sinensis. Crabs were exposed to ambient 0.00, 0.01, 0.05, 0.10, 0.20 and 0.30 mg/l Hg2+, and sampled on the days of 1, 7, 14 and 30 respectively. The results showed that both ambient Hg2+ concentrations and exposure time had significant effects on both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of E. sinensis, which showed the tendency to decrease with ambient mercury concentration increasing and exposure time prolonging. While for crabs exposed to 0.01 mg/l Hg2+, no significant difference was observed when compared with the control. Significant interaction between ambient Hg2+ and exposure time on these two ATPases activities was also observed with the lowest values found in crabs exposed to 0.30 mg/l Hg2+ for 30 days. The anterior gill microstructure analysis of E. sinensis exposed to different ambient Hg2+ for 30 days showed that mercury could alter the gill microstructures of E. sinensis. However, there was no significantly structure change appeared in crabs exposed to 0.01 mg/l Hg2+. Combined, these results indicated that though ambient Hg2+ could lead to significant decrease of both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, and could destroy the gill microstructures, the crab of E. sinensis could acclimate to low Hg2+ contamination less than 0.01mg/l.
Yanguo Shi and Jinlong Zuo
Y. M. Zhao et al., "Gill ATPase Activities and Microstructures of Juvenile Chinese Mitten Crab, Eriocheir sinensis, Exposed to Ambient Mercury", Advanced Materials Research, Vols. 183-185, pp. 1543-1548, 2011