Sulfate-reducing bacteria (SRB) was concentrated cultured and used to treat wastewater containing polyacrylimade. Viscosity loss was selected to indicate degradation of polyacrylimade. The experimental results show that SRB can be obtained from oil refinery wastewater. Suitable pH value, inoculation amount and polymer concentration for SRB were 7, 1% and 250mg/L to 400mg/L, respectively. The results also show that treatment of wastewater containing polymer by SRB is a perspective method.