Isolation and Dye Decolorization of a Bacillus subtilis Strain LS02 Exhibiting Laccase Activity

Mei Hui Du; Min Zhao; Lei Lu; Tian Nv Wang; Tai Lun Li; Li Yan Zhao; Jun Bo Pan; Guo Fu Li; Jun Li

doi:10.4028/www.scientific.net/AMR.183-185.839

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 183-185Isolation and Dye Decolorization of a Bacillus...

Isolation and Dye Decolorization of a Bacillus subtilis Strain LS02 Exhibiting Laccase Activity

Abstract:

A newly isolated strain LS02 was estimated for its ability in dye decolorization. The LS02 strain was identified as Bacillus subtilis by the combination of physicochemical tests and 16S rDNA sequence analysis. The isolated strain could oxidize the laccase substrate syringaldazine, indicating the existence of laccase activity. B. subtilis LS02 grown well in the pH range of 5.0~9.0, and showed an optimum growth temperature at 37°C. Indigo carmine could be completely decolorized by B. subtilis LS02 after 4 days, whereas Remazol Brilliant Blue R, reactive black 5 and crystal violet were poorly decolorized. The result indicated that the laccase of B. subtilis LS02 may be suitable for the application in textile bleaching of indigo carmine.

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Periodical:

Advanced Materials Research (Volumes 183-185)

Pages:

839-843

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.183-185.839

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Online since:

January 2011

Authors:

Mei Hui Du, Min Zhao, Lei Lu, Tian Nv Wang, Tai Lun Li, Li Yan Zhao, Jun Bo Pan, Guo Fu Li, Jun Li

Keywords:

Bacillus subtilis, Decolorization, Dye, Isolation, Laccase

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