This paper describes the laboratory measurement of shear interface properties between asphalt pavement and concrete bridge deck using the shear test. The main factors affecting shear strength are interface frictional resistance and adhesive stress from material of waterproof layer. Firstly, we simplify the whole system by considering the asphalt pavement as an elastic layer and the cement concrete deck a rigid base. And make a sensitivity analysis on interface contact state and the material modulus of waterproof layer. The maximum shear stress increases as interface contact state changes from continuous to smooth and as modulus of waterproof layer material decreases. Secondly, an interface shear experiment is conducted with the application of portable shear apparatus. With interface shear strength as evaluation indicator, shear strength of different interface treatments is arranged in descending order, i.e. cleaning up laitance and spreading aggregate between layers; only cleaning-up laitance; leaving the surface untreated. While the descending order for shear strength of different waterproof materials can be listed as follows: SBS modified asphalt, penetration grade of 70 asphalt. Therefore, based on the laboratory test results, it is recommended that before applying waterproof adhesive layer, laitance and dust on concrete deck surface should be cleaned up and keeping it dry. And the material for waterproof adhesive layer should be SBS modified asphalt with one-size aggregate of 4.75mm-9.5 mm spreading on.