Identification, Some Characterizations and Bioleaching Application of an Iron-Oxidizing Bacterium, Acidithiobacillus ferrooxidans KLipis-3-1 Isolated from Malaysia

Sei Choong Yee; Choon Ping Lim; Kim Yong Ng

doi:10.4028/www.scientific.net/AMR.20-21.453

Paper Titles

Differential Genetic Expression in Heap Bioleaching of Low-Grade Copper Sulphide Ore at Escondida Mine, Chile
p.435

Insights into the Metabolism and Ecophysiology of Three Acidithiobacilli by Comparative Genome Analysis
p.439

Involvement of Iron Oxidation Enzyme System in Sulfur Oxidation of Acidithiobacillus ferrooxidans ATCC 23270
p.443

The Effect of Total Iron Concentration and Iron Speciation on the Rate of Ferrous Iron Oxidation Kinetics of Leptospirillum ferriphilum in Continuous Tank Systems
p.447

Identification, Some Characterizations and Bioleaching Application of an Iron-Oxidizing Bacterium, Acidithiobacillus ferrooxidans KLipis-3-1 Isolated from Malaysia
p.453

Quantification of Biomining Microorganisms Using Quantitative Real-Time Polymerase Chain Reaction
p.457

Chalcopyrite and Bornite Differentially Affect Protein Synthesis in Planktonic Cells of Acidithiobacillus ferrooxidans
p.461

Temperature Effects on the Iron Oxidation Kinetics of a Leptospirillum ferriphilum Dominated Culture at pH Below One
p.465

Metabolism Peculiarities of Bacteria of the Genus Sulfobacillus
p.469

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Identification, Some Characterizations and Bioleaching Application of an Iron-Oxidizing Bacterium, Acidithiobacillus ferrooxidans KLipis-3-1 Isolated from Malaysia

Abstract:

A strain of At. ferrooxidans was isolated from Penjom Goldmine, Kuala Lipis, Pahang, Malaysia. The 16S rRNA partial gene sequence analysis revealed that this rod-shaped bacterium with size of 0.3 x 0.8 -m is a strain of At. ferrooxidans, and was designated as At. ferrooxidans KLipis-3-1. The whole-cell FAMEs profile analysis showed that At. ferrooxidans KLipis-3-1 possesses large amounts of C18:1, C16:0 and C16:1 fatty acids with C18:1 as the predominant fatty acid. The initial specific iron-oxidizing and molybdenum blue-oxidizing activities of this bacterium were 88.85 mMh-1mg-1 and 196.86 mMh-1mg-1, respectively, which were similar to the activities observed in the type strain At. ferrooxidans ATCC 23270. However, At. ferrooxidans KLipis-3-1 has a higher resistance towards Mo6+ at concentration above 1 mM. At. ferrooxidans KLipis-3-1 has the ability to reduce ferric ion with elemental sulfur as electron donor, with the initial specific SFORase activity of 0.47 mMh-1mg-1. The bioleaching capability of At. ferrooxidans KLipis-3-1 and At. ferrooxidans ATCC 23270 with pyrite as substrate at selected parameters operated in a batch bioprocess were studied.