Signal Processing Technique of Fluorescence Lifetime Imaging Using Four-Dimension Time Correlated Single Photon Counting Method

Cui Xia Sheng; He Jing Tang; Hong Yan Jia; Shu Lian Yang

doi:10.4028/www.scientific.net/AMR.216.350

Paper Titles

The Propagation Losses of Cold Deposited Zinc Sulfide Waveguides
p.332

Applying Template Method Pattern to Developing Electrochemical Workstation
p.337

Electronic Structure, Effective Masses and Optical Properties of Monoclinic HfO₂ from First-Principles Calculations
p.341

Research on Milling Spiral Bevel Gear Using CТС Machine Tool
p.345

Signal Processing Technique of Fluorescence Lifetime Imaging Using Four-Dimension Time Correlated Single Photon Counting Method
p.350

Research on the Photoelectric Conversion Efficiency of Grating Anti-Reflective Layer Solar Cells
p.355

Realization of Wireless Communication Card Based on UWB for Industrial Internet of Things
p.360

A Delay Optimization of Non-Periodic Information for the Scheduling Algorithm
p.364

Dependence of Transient Current and Capacitance of Organic Light-Emitting Diodes on Pulsed Bias Conditions
p.368

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 216Signal Processing Technique of Fluorescence...

Signal Processing Technique of Fluorescence Lifetime Imaging Using Four-Dimension Time Correlated Single Photon Counting Method

Abstract:

Measurement of fluorescence lifetime plays an important role in fluorescence lifetime imaging technology. If the Time correlated single photon counting method is used to process the signal of fluorescence lifetime images, it can make the real time imaging which have the virtue of high timing resolve and high signal to noise ratio. Multi-wavelength channels are used to record the lifetime simultaneously in four-dimension TCSPC system design. Four-dimension TCSPC imaging can be used to record the photon density over time, wavelength and coordinates of the scanning area. Analysis shows that the counting efficiency and the amount of information in the data can be increased by recording the fluorescence in several wavelength channels simultaneously. Multi-wavelength imaging was also successfully used to separate different fluorephores in stead-state images.

You might also be interested in these eBooks

Optical, Electronic Materials and Applications

View Preview

Info:

Periodical:

Advanced Materials Research (Volume 216)

Pages:

350-354

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.216.350

Citation:

Cite this paper

Online since:

March 2011

Authors:

Cui Xia Sheng, He Jing Tang, Hong Yan Jia, Shu Lian Yang

Keywords:

Fluorescence Lifetime, Four-Dimension TCSPC, Multi-Channel Detection, TCSPC Imaging

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Wolfgang Becker, Axel Bergmann, Elke Haustein, Fluorescence lifetime images and correlation spectra obtained by multi-dimensional TCSPC ,. Proc. SPIE 5700, (2005).

Google Scholar

[2] Kirsten ALTENBACH, Rory R. DUNCANc, Mari VALKONEN., In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi, Fungal Biology Reviews. no. 23, p.67 –71 (2009).

DOI: 10.1016/j.fbr.2009.12.002

Google Scholar

[3] Qu J L, Lin Z Y, et al., Fluorescence Lifetime Imaging and Its Biomedical Applications, Journal of Shenzhen University Science and Engineering. Vol. 22, no. 2, pp.133-141 (2005).

Google Scholar

[4] Wolfgang Becker, Axel Bergmann, Christoph Biskup, et al, Multiwave length TCSPC lifetime imaging, Proc. SPIE 4620, pp.79-84 (2002).

Google Scholar

[5] ZHANG Xiu-feng , SONG Ke-fei , YU Tao, et al, The Key Technology of Picosecond Time-Correlated Single Photon Counting Spectrometer, Spectroscopy and Spectral Analysis. vol. 24, no. 12, pp.1489-1493 (2004).

Google Scholar

[6] Erik B, van Munster , Theodorus W, et al , Fluorescence Lifetime Imaging Microscopy (FLIM) , Adv Biochem Engin/Biotechnol. no. 95, p.143–175 (2005).

Google Scholar