Magnetic P(St-MA) Microspheres：Preparation, Characterization and Interaction with BSA

Abstract:

Taken the nanoparticles Fe3O4 as the magnetic carrier, and styrene ( St ) and Methyl Acrylate (MA) as the monomers, the magnetic polystyrene-methyl acrylate (P(St-MA)) microspheres were prepared by microsuspension copolymerization. The effect of various parameters(e.g. reaction temperature, initiator, Fe3O4/styrene ratio and disperser) on the properties of the P(St-MA) microspheres were studied.The microspheres were characterized by different techniques (e.g. SEM, VSM, TG-DSC, FTIR, ). The interaction of magnetic microspheres and BSA were studied by microcalorimetry. The results showed the P(St-MA) microspheres, with abundant –COOH, superparamagnetic and high magnetic content, were possessed much higher protein adsorbance compared to those of magnetic polystyrene(PS) microspheres.

• Periodical: Advanced Materials Research (Volumes 217-218)
• Pages: 249-255
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.217-218.249
• Online since: March 2011
• Authors: Ling Ding, Xi Li, Chao Can Zhang
• Keywords: Adsorption Capacity, Adsorption Heat, BSA, Magnetic P(St-MA) Microspheres, Microcalorimetry, Microsuspension Polymerization

References

[1] G. H. Qiu, Q. Wang, C. Wang and Y. L. Guo: Ultrason. Sonochem. Vol. 14(2007), pp.55-61.

[2] Z. Z. Xu, A. Xia, C.C. Wang and W.L. Yang: Mater. Chem. Phys. Vol. 103(2007), pp.494-499.

[3] U. Akiva, and S. Margel: J. Colloid. Interf. Sci. Vol. 288( 2005), pp.61-70.

[4] J. Zhang, S.T. Zhang and Y.P. Wang: J. Magn. Magn. Mater. Vol. 309(2007), pp.197-201.

[5] A. Spanova, B. Rittich, and I. Styriakova: J. Chromatogr. A. Vol. 1130(2006), pp.115-121.

[6] V.R. Sinha, A.K. Singla, S. Wadhawan and R. Kaushik: Int. J. Pharm. Vol. 274(2004), pp.1-33.

[7] L. R. Moore, M. Zborowski, M. Nakamura, K. McCloskey, S. Gura, M. Zuberi, S. Margel and J. J. Chalmers: J. Biochem. Biophy. Meth. Vol. 44( 2000), pp.115-130.

DOI: 10.1016/s0165-022x(00)00085-3

[8] S. L. Wang, Q. B. Wang, H. C. Gu and Y. H. Zhu: J. Chem. World. Vol. 7 (2001), pp.384-386.

[9] X. Li, Y.Q. Jin, L. Ding and C.C. Zhang: J. Func Mater. Vol. 35(2004), pp.2780-2783.

[10] Z. L. Liu and X. B. Yang: J. Huazhong Univ. of Sci. and Tech. Vol. 34(2006), pp.46-48.

[11] L. Ding, X. Li and C.C. Zhang: Chem. & Bioengineering, Vol. 25(2008), pp.63-68.

[12] L. Ding, X. Li and C.C. Zhang: Chem. Research, Vol. 21 (2010), pp.19-22.

[13] P. Liu, X. Li and M. Pan: Acta. Phy. Chim. Sin. Vol. 24 (2008), pp.161-164.

[14] M. Riza, S. Tokura, M. Iwasaki and E. Yashima: J Polym Sci. Vol. 33(1995), pp.1219-1225.