This paper studied the green finishing methods on cotton fabric. It discussed the effects of wear performance with enzyme treatment and chitosan finishing on the cotton fabrics. Cotton fabrics were treated by amylase LA, the soft, smooth of the fabric improved and the tensile strength decreased. When the temperature of amylase LA in 30°C ~ 70°C, its activity and starch slurry removal rate increased with the temperature increasing. Above 70°C, activity decreased, so the temperature herein amylase optimum treatment temperature is 70°C. After the enzymatic treatment, and then by chitosan finishment, the results show that after treating, the hand, anti-wrinkle, anti-bacteria and other performance of the fabrics have greatly improved.