Determination of Stigmasterol in Jatropha Seed Oil by High Performance Liquid Chromatography

Shu Yu Liu; Li Jie Sun; Xin Guo

doi:10.4028/www.scientific.net/AMR.233-235.1206

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 233-235Determination of Stigmasterol in Jatropha Seed Oil...

Determination of Stigmasterol in Jatropha Seed Oil by High Performance Liquid Chromatography

Abstract:

A simple and rapid high performance liquid chromatography (HPLC) assay was developed to identify and measure the stigmasterol in jatropha seed oil. The stigmasterol was isolated with a good selectivity by HPLC employing reversed phase C18 columns. The components were separated by mobile phase of methanol-water (99/1, v/v) and detected at 202nm. The quantitation of the stigmasterol was reproducible and the method relative standard deviation is 1.6%. The mean analytical recovery was 97.0%．