N-(2-pyridylmethyl)-L-phenylalanine methyl ester was synthesized in our laboratory，and the title compound was synthesized by the reaction of N-(2-pyridylmethyl)-L-phenylalanine methyl ester and Zinc acetate dehydrate. Bis(L-α-N-(2-pyridylmethy)phenylalaninato-N, N', O)-Zinc(II) trihydrate was prepared and structurally characterized by X-ray diffraction. It crystallizes in orthorhombic, space group P21212 with a= 15.0928 (8), b = 16.4209 (6), c = 5.9930 (3) Å, V = 1485.29 (12) Å3, Z = 2, Mr = 630.00, Dx = 1.409 g/cm3, μ = 0.88 mm-1, F(000) = 660, Rint = 0.0205, R = 0.028 and wR = 0.059 for 2943 observed reflections with I > 2σ(I). It is a mononuclear discrete structure. The zinc atom coordinates with two oxygen and four nitrogen atoms from two ligands in a distorted octahedral geometry, with the oxygen atom from the carboxylate, two of nitrogen atoms from NH parts and the other two nitrogen atoms from the pyridine ring. With three free water molecules, the present structure has a network of hydrogen bonds.